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PCC-Pinboard

PCC is closed until July 7th 2020.
The opening hours can be found in the Crystallization Calendar



New screen:

The Small Molecule Anion screen developed by Prof. Dr. Bernhard Spingler (UZH) is now available as GS086 .

Crystal Screen 1+2 Cryo GS084
Hampton Research screen detail: HR2-122 + HR2-121

The Ammonium sulfate screen GS002 has been newly designed and is now available as GS085.


What’s new:


What's New in ROCK MAKER 3.15? Protein Crystallization Software Update ROCK MAKER® 3.15

Contact

Department of Biochemistry
University of Zurich
Winterthurerstr. 190
CH-8057 Zurich
Office: Y44 J 30/34
xtal@bioc.uzh.ch

Beat Blattmann
Tel.: +41 44 635 6557
Caroline Müller
Tel.: +41 44 635 5548

Sample Volume

Vapor diffusion crystallization experiment

The Phoenix and Gryphon-LCP liquid handling system can routinely dispense a drop volume between 100nl and 250nl for both the protein sample and reservoir solution into the well of a MRC 3-lens crystallization plate. Up to three different protein samples can be dispensed on a single MRC 3-lens crystallization plate. The protein sample volume needed for the crystallization experiments depends on the drop volume and the number of plates:


Drop volume Sample volume per plateSample volume for N plates
100 nl9.6 ulN*9.6 +20 ul
150 nl14.4 ulN*14.4 +20 ul
.........
250 nl24.0 ulN*24.0 +20 ul

Example of sample volume calculation:

The sample volume required for 4 plates (96 Well), 3 drops of (100nl, 150nl, 200nl)  is:
(4 x (0.1+0.15+0.2)uL x 96) +20 =192.8 uL


Note:

Left-over protein samples not used for crystallization will be stored at 4°C for up to one month. Samples older than 1 month will be discharged without notice.

Microseeding crystallization experiment

Seedstock drop volumeSeedstock volume per plateSeedstock volume for N plate
20 nl>2.5 ulN*2.5 +20 ul
50 nl5.0 ulN*5.0 +20 ul

LCP-Experiments

Protein sample volumeCubic phase drop volumeMax. # of plates
40 ul50 nl18 plates
40 ul100 nl9 plates
40 ul150 nl6 plates
20 ul50 nl10 plates
20 ul100 nl5 plates
20 ul150 nl3 plates