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New screen:

Crystal Screen 1+2 Cryo GS084
Hampton Research screen detail: HR2-122 + HR2-121

The Ammonium sulfate screen GS002 has been newly designed and is now available as GS085.


What’s new:


What's New in ROCK MAKER 3.15? Protein Crystallization Software Update ROCK MAKER® 3.15

Please note that for the coming weeks PCC will be closed all day Mondays, Tuesdays and Friday morning until further notice. 19.11.2019

Contact

Department of Biochemistry
University of Zurich
Winterthurerstr. 190
CH-8057 Zurich
Office: Y44 J 30/34
xtal@bioc.uzh.ch

Beat Blattmann
Tel.: +41 44 635 6557
Caroline Müller
Tel.: +41 44 635 5548

Sample Volume

Vapor diffusion crystallization experiment

The Phoenix and Gryphon-LCP liquid handling system can routinely dispense a drop volume between 100nl and 250nl for both the protein sample and reservoir solution into the well of a MRC 3-lens crystallization plate. Up to three different protein samples can be dispensed on a single MRC 3-lens crystallization plate. The protein sample volume needed for the crystallization experiments depends on the drop volume and the number of plates:


Drop volume Sample volume per plateSample volume for N plates
100 nl9.6 ulN*9.6 +20 ul
150 nl14.4 ulN*14.4 +20 ul
.........
250 nl24.0 ulN*24.0 +20 ul

Example of sample volume calculation:

The sample volume required for 4 plates (96 Well), 3 drops of (100nl, 150nl, 200nl)  is:
(4 x (0.1+0.15+0.2)uL x 96) +20 =192.8 uL


Note:

Left-over protein samples not used for crystallization will be stored at 4°C for up to one month. Samples older than 1 month will be discharged without notice.

Microseeding crystallization experiment

Seedstock drop volumeSeedstock volume per plateSeedstock volume for N plate
20 nl>2.5 ulN*2.5 +20 ul
50 nl5.0 ulN*5.0 +20 ul

LCP-Experiments

Protein sample volumeCubic phase drop volumeMax. # of plates
40 ul50 nl18 plates
40 ul100 nl9 plates
40 ul150 nl6 plates
20 ul50 nl10 plates
20 ul100 nl5 plates
20 ul150 nl3 plates