Structure Gallery 2014

The following structures have been solved using our high throughput crystallization services:

Specific targeting of human caspases using designed ankyrin repeat proteins.
Flütsch A, Schroeder T, Barandun J, Ackermann R, Bühlmann M, Grütter MG.
Biol Chem. 2014 Oct;395(10):1243-52
PDB: 2Y1L

 

Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter.
Hohl M, Hürlimann LM, Böhm S, Schöppe J, Grütter MG, Bordignon E, Seeger MA.
Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):11025-30.
PDB ID: 4Q4A (with bound AMP-PNP), 4Q4H (apo), 4Q4J (cross-linked D-loops)

 

Phase Behavior of a Designed Cyclopropyl Analogue of Monoolein: Implications for Low-Temperature Membrane Protein Crystallization.;Salvati Manni L, Zabara A, Osornio YM, Schöppe J, Batyuk A, Plückthun A, Siegel JS, Mezzenga R, Landau EM. Angew Chem Int Ed Engl. 2014 Nov 21.

 

X-ray structure of a calcium-activated TMEM16 lipid scramblase.
Brunner JD, Lim NK, Schenck S, Duerst A, Dutzler R.
Nature. 2014 Nov 12. doi: 10.1038/nature13984
PDB ID: 4WIT4WIS

 

Crystal structures of designed armadillo repeat proteins: Implications of construct design and crystallization conditions on overall structure.
Reichen C, Madhurantakam C, Plückthun A, Mittl PR.
Protein Sci. 2014 Aug 13.
PDB ID: 4PLR.

 

Characterization of Variants of the Pore-Forming Toxin ClyA from Escherichia coli Controlled by a Redox Switch
Daniel Roderer, Stephan Benke, Marcus Müller, Helene Fäh-Rechsteiner, Nenad Ban, Benjamin Schuler, and Rudi Glockshuber
Biochemistry. 2014 Oct 14;53(40):6357-69
PDB ID: 4PHO and 4PHQ

 

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport
Ines A Ehrnstorfer, Eric R Geertsma, Els Pardon, Jan Steyaert & Raimund Dutzler
Nat Struct Mol Biol. 2014 October 19, doi: 10.1038/nsmb.2904
PDB ID: 4WGV and 4WGW

 

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease.
Anders C, Niewoehner O, Duerst A, Jinek M.
Nature. 2014 Jul 27.
PDB ID:4un34un4 and 4un5

 

Co-Crystallization with Conformation-Specific Designed Ankyrin Repeat Proteins Explains the Conformational Flexibility of BCL-W.
Schilling J, Schöppe J, Sauer E, Plückthun A.
J Mol Biol. 2014 Jun 12;426(12):2346-62.
PDB ID: 4K5A and 4K5B

 

Structural basis of substrate specificity of human oligosaccharyl transferase subunit N33/Tusc3 and its role in regulating protein N-glycosylation.
Mohorko E, Owen RL, Malojčić G, Brozzo MS, Aebi M, Glockshuber R.
Structure. 2014 Apr 8;22(4):590-601.
PDB ID: 4M8G4M904M91, and 4M92..

 

Combined inhibition of caspase-3 and caspase-7 by two highly selective DARPins slows down cellular demise.
Flütsch A, Ackermann R, Schroeder T, Lukarska M, Hausammann GJ, Weinert C, Briand C, Grütter MG.
Biochem J. 2014 Apr 30.
PDB ID: 4LSZ

 

Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli.
Egloff P, Hillenbrand M, Klenk C, Batyuk A, Heine P, Balada S, Schlinkmann KM, Scott DJ, Schütz M, Plückthun A.
Proc Natl Acad Sci U S A. 2014 Jan 22.
PDB ID:4BUO