Structure Gallery 2018

The following structures have been solved using our high throughput crystallization services:

Structure of a volume-regulated anion channel of the LRRC8 family.
Dawid Deneka, Marta Sawicka, Andy K. M. Lam, Cristina Paulino & Raimund Dutzler.
Nature 558, 254–259 (2018);

Evolution of a highly active and enantiospecific metalloenzyme from short peptides.
Sabine Studer, Douglas A. Hansen, Zbigniew L. Pianowski, Peer R. E. Mittl, Aaron Debon, Sharon L. Guffy, Bryan S. Der, Brian Kuhlman, Donald Hilvert.
Science, 14 Dec 2018: Vol. 362, Issue 6420, pp. 1285-1288; doi: 10.1126/science.aau3744

High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist.
Ehrenmann J, Schöppe J, Klenk C, Rappas M, Kummer L, Doré AS, Plückthun A.
Nat Struct Mol Biol. 2018 Dec;25(12):1086-1092. doi: 10.1038/s41594-018-0151-4. Epub 2018 Nov 19.

Design of ultra-swollen lipidic mesophases for the crystallization of membrane proteins with large extracellular domains.
Zabara A, Chong JTY, Martiel I, Stark L, Cromer BA, Speziale C, Drummond CJ, Mezzenga R.
Nat Commun. 2018 Feb 7; 9: 544. doi: 10.1038/s41467-018-02996-5

Structural Basis for the Selective Inhibition of c-Jun N-Terminal Kinase 1 Determined by Rigid DARPin-DARPin Fusions.
Wu Y, Honegger A, Batyuk A, Mittl PRE, Plückthun A.
J Mol Biol. 2018 Jul 6;430(14):2128-2138. doi: 10.1016/j.jmb.2017.10.032. Epub 2017 Nov 8.

Curvature of designed armadillo repeat proteins allows modular peptide binding.
Hansen S, Ernst P, König SLB, Reichen C, Ewald C, Nettels D, Mittl PRE, Schuler B, Plückthun A.
J Struct Biol. 2018 Feb;201(2):108-117. doi: 10.1016/j.jsb.2017.08.009. Epub 2017 Aug 31.

An Interface-Driven Design Strategy Yields a Novel, Corrugated Protein Architecture.
ElGamacy M, Coles M, Ernst P, Zhu H, Hartmann MD, Plückthun A, Lupas AN.
ACS Synth Biol. 2018 Sep 21;7(9):2226-2235. doi: 10.1021/acssynbio.8b00224. Epub 2018 Sep 4.

The extracellular gate shapes the energy profile of an ABC exporter.
Hutter CAJ, Timachi MH, Hürlimann LM, Zimmermann I, Egloff P, Göddeke H, Kucher S, Štefanić S, Karttunen M, Schäfer LV, Bordignon E, Seeger MA.
bioRxiv. 2018 Sep 18; 421073; doi: 10.1101/421073

Synthetic single domain antibodies for the conformational trapping of membrane proteins.
Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA.
Elife. 2018 May 24;7. pii: e34317. doi: 10.7554/eLife.34317.

A high throughput screening method for the nano-crystallization of salts of organic cations.
Nievergelt PP, Babor M, Čejka J, Spingler B.
Chem Sci. 2018 Apr 21; 9(15): 3716–3722.Published online 2018 Mar 12. doi:  10.1039/c8sc00783g

Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control.
Schmid M, Ernst P, Honegger A, Suomalainen M, Zimmermann M, Braun L, Stauffer S, Thom C, Dreier B, Eibauer M, Kipar A, Vogel V, Greber UF, Medalia O, Plückthun A.
Nat Commun. 2018 Jan 31;9(1):450. doi: 10.1038/s41467-017-02707-6.

Nonribosomal biosynthesis of backbone-modified peptides.
Niquille DL, Hansen DA, Mori T, Fercher D, Kries H, Hilvert D.
Nat Chem. 2018 Mar;10(3):282-287. doi: 10.1038/nchem.2891. Epub 2017 Nov 20.

Structural basis for ion selectivity in TMEM175 K+ channels.
Brunner JD, Jakob RP, Schulze T, Neldner Y, Moroni A, Thiel G, Maier T, Schenck S.
bioRxiv. 2018 Dec 4; 480863; doi: 10.1101/480863

Rational Engineering of a Designed Protein Cage for siRNA Delivery.
Edwardson TGW, Mori T, Hilvert D.
J Am Chem Soc. 2018 Aug 22;140(33):10439-10442. doi: 10.1021/jacs.8b06442. Epub 2018 Aug 9.

A Noncanonical Proximal Heme Ligand Affords an Efficient Peroxidase in a Globin Fold.
Pott M, Hayashi T, Mori T, Mittl PRE, Green AP, Hilvert D.
J Am Chem Soc. 2018 Jan 31;140(4):1535-1543. doi: 10.1021/jacs.7b12621. Epub 2018 Jan 19.