Publications on Repeat Proteins (DARPins and others)

Work on Repeat Proteins

Click the number of a publication to read its abstract. For some of the publications supplementary files are available on the abstract page.

Number Reference PDF
0453 Mittl, P.R., Ernst P. and Plückthun, A. (2020). Chaperone-assisted structure elucidation with DARPins. Curr. Opin. Struct. Biol. 60, 93-100.
0450 Balakrishnan, A., Rajan, A., Salter, A. I., Kosasih, P. L., Wu, Q., Voutsinas, J., Jensen, M. C., Plückthun, A. and Riddell, S. R. (2019). Multispecific Targeting with Synthetic Ankyrin Repeat Motif Chimeric Antigen Receptors. Clin. Cancer Res. , 7506-7516.
0449 Chernyavska, M., Schmid, M., Freitag, P. C., Palacio-Castaneda, V., Piruska, A., Huck, W. T. S., Plückthun, A. and Verdurmen, W. P. R. (2019). Unravelling receptor and RGD motif dependence of retargeted adenoviral vectors using advanced tumor model systems. Sci Rep. 9, 18568.
0448 Ernst, P., Honegger, A., van der Valk, F., Ewald, C., Mittl, P. R. E., Plückthun, A. (2019). Rigid fusions of designed helical repeat binding proteins efficiently protect a binding surface from crystal contacts. Sci Rep. 9, 16162.
0446 Ernst, P., Plückthun, A., and Mittl, P. R. E. (2019). Structural analysis of biological targets by host:guest crystal lattice engineering. Sci. Rep. 9, 15199.
0445 Renko, M., Fiedler, M., Rutherford, T. J., Schaefer, J. V., Plückthun, A., and Bienz, M. (2019). Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome. Proc. Natl. Acad. Sci. U. S. A. 116, 20977-20983.
0443 Brandl, F., Merten, H., Zimmermann, M., Behe, M., Zangemeister-Wittke, U., and Plückthun, A. (2019). Influence of size and charge of unstructured polypeptides on pharmacokinetics and biodistribution of targeted fusion proteins. J. Control. Release 307, 379-392.
0442 Merten, H., Schaefer, J. V., Brandl, F., Zangemeister-Wittke, U., and Plückthun, A. (2019). Facile site-specific multiconjugation strategies in recombinant proteins produced in bacteria. Methods Mol. Biol. 2033, 253-273.
0441 Zhang, Y., Auger, S., Schaefer, J. V., Plückthun, A., and Distefano, M. D. (2019). Site-selective enzymatic labeling of designed ankyrin repeat proteins using protein farnesyltransferase. Methods Mol. Biol. 2033, 207-219.
0440 Stüber, J. C., Kast, F., and Plückthun, A. (2019). High-throughput quantification of surface protein internalization and degradation. ACS Chem. Biol. 14, 1154-1163.
0439 Radom, F., Paci, E., and Plückthun, A. (2019). Computational modeling of designed ankyrin repeat protein complexes with their targets. J. Mol. Biol. 431, 2852-2868.
0438 Lorenzer, C., Streussnig, S., Tot, E., Winkler, A. M., Merten, H., Brandl, F., Sayers, E. J., Watson, P., Jones, A. T., Zangemeister-Wittke, U., Plückthun, A., and Winkler, J. (2019). Targeted delivery and endosomal cellular uptake of DARPin-siRNA bioconjugates: Influence of linker stability on gene silencing. Eur. J. Pharm. Biopharm. 141, 37-50.
0437 Campanacci, V., Urvoas, A., Cantos-Fernandes, S., Aumont-Nicaise, M., Arteni, A. A., Velours, C., Valerio-Lepiniec, M., Dreier, B., Plückthun, A., Pilon, A., Pous, C., Minard, P., and Gigant, B. (2019). Insight into microtubule nucleation from tubulin-capping proteins. Proc. Natl. Acad. Sci. U. S. A. 116, 9859-9864.
0436 Meksiriporn, B., Ludwicki, M. B., Stephens, E. A., Jiang, A., Lee, H. C., Waraho-Zhmayev, D., Kummer, L., Brandl, F., Plückthun, A., and DeLisa, M. P. (2019). A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins. Nature communications 10, 1830.
0435 Andres, F., Iamele, L., Meyer, T., Stüber, J. C., Kast, F., Gherardi, E., Niemann, H. H., and Plückthun, A. (2019). Inhibition of the MET kinase activity and cell growth in MET-addicted cancer cells by bi-paratopic linking. J. Mol. Biol. 431, 2020-2039.
0432 Michel, E., Plückthun, A., and Zerbe, O. (2019). Peptide binding affinity redistributes preassembled repeat protein fragments. Biol. Chem. 400, 395-404.
0431 Schwill, M., Tamaskovic, R., Gajadhar, A. S., Kast, F., White, F. M., and Plückthun, A. (2019). Systemic analysis of tyrosine kinase signaling reveals a common adaptive response program in a HER2-positive breast cancer. Science Signaling12, eaau2875.
0427 Vigano, M. A., Bieli, D., Schaefer, J. V., Jakob, R. P., Matsuda, S., Maier, T., Plückthun, A., and Affolter, M. (2018). DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations. Biology Open 7, bio036749. doi:10.1242/bio.036749
0426 ElGamacy, M., Coles, M., Ernst, P., Zhu, H., Hartmann, M. D., Plückthun, A., and Lupas, A. N. (2018). An Interface-driven design strategy yields a novel, corrugated protein architecture. ACS Synth. Biol. 7, 2226-2235.
0423 Mikula, K. M., Krumwiede, L., Plückthun, A., and Iwai, H. (2018). Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation. J. Biomol. NMR 71, 225-235.
0422 Hartmann, J., Münch, R. C., Freiling, R. T., Schneider, I. C., Dreier, B., Samukange, W., Koch, J., Seeger, M. A., Plückthun, A., and Buchholz, C. J. (2018). A library-based screening strategy for the identification of DARPins as ligands for receptor-targeted AAV and lentiviral vectors. Mol. Ther. Methods Clin. Dev. 10, 128-143.
0420 Radom, F., Plückthun, A., and Paci, E. (2018). Assessment of ab initio models of protein complexes by molecular dynamics. PLoS Comput. Biol. 14, e1006182; DOI:10.1371/journal.pcbi.1006182
0419 Wu, Y., Honegger, A., Batyuk, A., Mittl, P. R. E., and Plückthun, A. (2018). Structural basis for the selective inhibition of c-Jun N-terminal kinase 1 determined by rigid DARPin-DARPin fusions. J. Mol. Biol. 430, 2128-2138.
0417 Michel, E., Plückthun, A., and Zerbe, O. (2018). Peptide-guided assembly of repeat protein fragments. Angew. Chem. Int. Ed. Engl. 57, 4576-4579.
0415 Hansen, S., Ernst, P., König, S. L. B., Reichen, C., Ewald, C., Nettels, D., Mittl, P., Schuler, B., and Plückthun, A. (2018). Curvature of designed armadillo repeat proteins allows modular peptide binding. J. Struct. Biol. 201, 108-117.
0414 Schmid, M., Ernst, P., Honegger, A., Suomalainen, M., Zimmermann, M., Braun, L., Stauffer, S., Thom, C., Dreier, B., Eibauer, M., Kipar, A., Vogel, V., Greber, U. F., Medalia, O., and Plückthun, A. (2018). Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control. Nature Communications 9, 450; DOI:10.1038/s41467-017-02707-6
0413 Hansen, S., Stüber, J. C., Ernst, P., Koch, A., Bojar, D., Batyuk, A., and Plückthun, A. (2017). Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity. Sci. Rep. 7, 16292; DOI:10.1038/s41598-017-15711-z
0411 Verdurmen, W. P. R., Mazlami, M., and Plückthun, A. (2017). A quantitative comparison of cytosolic delivery via different protein uptake systems. Sci. Rep 7, 13194; 10.1038/s41598-017-13469-y
0410 Hansen, S., Kiefer, J. D., Madhurantakam, C., Mittl, P. R. E., and Plückthun, A. (2017). Structures of designed armadillo repeat proteins binding to peptides fused to globular domains. Protein Sci. 26, 1942-1952.
0409 Wu, Y., Batyuk, A., Honegger, A., Brandl, F., Mittl, P., and Plückthun, A. (2017). Rigidly connected multispecific artificial binders with adjustable geometries. Sci. Rep. 7, 11217; DOI:10.1038/s41598-017-11472-x
0408 Jost, C., Stüber, J. C., Honegger, A., Wu, Y., Batyuk, A., and Plückthun, A. (2017). Rigidity of the extracellular part of HER2: Evidence from engineering subdomain interfaces and shared-helix DARPin-DARPin fusions. Protein Sci. 26, 1796-1806.
0403 Verdurmen, W. P., Mazlami, M., and Plückthun, A. (2017). A biotin ligase-based assay for the quantification of the cytosolic delivery of therapeutic proteins. Methods Mol. Biol. 1575, 223-236.
0402 Anders, U., Schaefer, J. V., Hibti, F. E., Frydman, C., Suckau, D., Plückthun, A., and Zenobi, R. (2017). SPRi-MALDI MS: characterization and identification of a kinase from cell lysate by specific interaction with different designed ankyrin repeat proteins. Anal. Bioanal. Chem. 409, 1827-1836.
0401 Syafrizayanti, Lueong, S. S., Di, C., Schaefer, J. V., Plückthun, A., and Hoheisel, J. D. (2017). Personalised proteome analysis by means of protein microarrays made from individual patient samples. Sci. Rep. 7, 39756; DOI: 10.1038/srep39756
0399 Ernst, P., and Plückthun, A. (2017). Advances in the design and engineering of peptide-binding repeat proteins. Biol. Chem. 398, 23-29.
0398 Reichen, C., Hansen, S., Forzani, C., Honegger, A., Fleishman, S. J., Zhou, T., Parmeggiani, F., Ernst, P., Madhurantakam, C., Ewald, C., Mittl, P. R., Zerbe, O., Baker, D., Caflisch, A., and Plückthun, A. (2016). Computationally designed armadillo repeat proteins for modular peptide recognition. J. Mol. Biol. 428, 4467-4489
0397 Ahmad, S., Pecqueur, L., Dreier, B., Hamdane, D., Aumont-Nicaise, M., Plückthun, A., Knossow, M., and Gigant, B. (2016). Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin. Sci. Rep. 6, 28922; doi: 10.1038/srep28922.
0395 Bender, R. R., Muth, A., Schneider, I. C., Friedel, T., Hartmann, J., Plückthun, A., Maisner, A., and Buchholz, C. J. (2016). Receptor-targeted Nipah virus glycoproteins improve cell-type selective gene delivery and reveal a preference for membrane-proximal cell attachment. PLoS Pathog. 12, e1005641; doi: 10.1371/journal.ppat.1005641
0394 Tamaskovic, R., Schwill, M., Nagy-Davidescu, G., Jost, C., Schaefer, D. C., Verdurmen, W. P. R., Schaefer, J. V., Honegger, A., and Plückthun, A. (2016). Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS–p110 crosstalk. Nature Commun. 7, 11672; doi: 10.1038/ncomms11672.

0393 Batyuk, A., Wu, Y., Honegger, A., Heberling, M. M., and Plückthun, A. (2016). DARPin-based crystallization chaperones exploit molecular geometry as a screening dimension in protein crystallography. J. Mol. Biol. 428, 1574-1588.
0392 Schütz, M., Batyuk, A., Klenk, C., Kummer, L., de Picciotto, S., Gülbakan, B., Wu, Y., Newby, G. A., Zosel, F., Schöppe, J., Sedlàk, E., Mittl, P. R., Zenobi, R., Wittrup, K. D., and Plückthun, A. (2016). Generation of fluorogen-activating Designed Ankyrin Repeat Proteins (FADAs) as versatile sensor tools. J. Mol. Biol. 428, 1272-1289.
0391 Hansen, S., Tremmel, D., Madhurantakam, C., Reichen, C., Mittl, P. R., and Plückthun, A. (2016). Structure and energetic contributions of a designed modular peptide-binding protein with picomolar affinity. J. Am. Chem. Soc. 138, 3526-3532.
0389 Hanauer, J. R., Gottschlich, L., Riehl, D., Rusch, T., Koch, V., Friedrich, K., Hutzler, S., Prüfer, S., Friedel, T., Hanschmann, K. M., Münch, R. C., Jost, C., Plückthun, A., Cichutek, K., Buchholz, C. J., and Mühlebach, M. D. (2016). Enhanced lysis by bispecific oncolytic measles viruses simultaneously using HER2/neu or EpCAM as target receptors. Mol. Ther. 3, 16003; doi:10.1038/mto.2016.3.
0386 Reichen, C., Madhurantakam, C., Hansen, S., Grütter, M. G., Plückthun, A., and Mittl, P. R. (2016). Structures of designed armadillo-repeat proteins show propagation of inter-repeat interface effects. Acta Crystallogr. D72, 168-175.
0384 Merten, H., Brandl, F., Plückthun, A., and Zangemeister-Wittke, U. (2015). Antibody-drug conjugates for tumor targeting-novel conjugation chemistries and the promise of non-IgG binding proteins. Bioconjug. Chem. 26, 2176-2185.
0381 Ewald, C., Christen, M. T., Watson, R. P., Mihajlovic, M., Zhou, T., Honegger, A., Plückthun, A., Caflisch, A., and Zerbe, O. (2015). A combined NMR and computational approach to investigate peptide binding to a designed armadillo repeat protein. J. Mol. Biol. 427, 1919-1933.
0377 Oude Blenke, E., Klaasse, G., Merten, H., Plückthun, A., Mastrobattista, E., and Martin, N. I. (2015). Liposome functionalization with copper-free "click chemistry". J. Control. Release 202, 14-20.
0376 Münch, R. C., Muth, A., Muik, A., Friedel, T., Schmatz, J., Dreier, B., Trkola, A., Plückthun, A., Büning, H., and Buchholz, C. J. (2015). Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors. Nat. Commun. 6, 6246.
0375 Goldstein, R., Sosabowski, J., Livanos, M., Leyton, J., Vigor, K., Bhavsar, G., Nagy-Davidescu, G., Rashid, M., Miranda, E., Yeung, J., Tolner, B., Plückthun, A., Mather, S., Meyer, T., and Chester, K. (2015). Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging. Eur. J. Nucl. Med. Mol. Imaging 42, 288-301.
0372 Plückthun, A. (2015). Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy. Annu. Rev. Pharmacol. Toxicol. 55, 489-511.
0371 Verdurmen, W. P., Luginbühl, M., Honegger, A., and Plückthun, A. (2015). Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems. J. Control. Release 200, 13-22.
0370 Stefan, N., Zimmermann, M., Simon, M., Zangemeister-Wittke, U., and Plückthun, A. (2014). Novel prodrug-like fusion toxin with protease-sensitive bioorthogonal PEGylation for tumor targeting. Bioconjug. Chem. 25, 2144-2156.
0369 Brauchle, M., Hansen, S., Caussinus, E., Lenard, A., Ochoa-Espinosa, A., Scholz, O., Sprecher, S. G., Plückthun, A., and Affolter, M. (2014). Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry. Biol. Open 3, 1252-1261.
0368 Reichen, C., Madhurantakam, C., Plückthun, A., and Mittl, P. R. (2014). Crystal structures of designed armadillo repeat proteins: Implications of construct design and crystallization conditions on overall structure. Protein Sci. 23, 1572-1583.
0367 Hanenberg, M., McAfoose, J., Kulic, L., Welt, T., Wirth, F., Parizek, P., Strobel, L., Cattepoel, S., Spani, C., Derungs, R., Maier, M., Plückthun, A., and Nitsch, R. M. (2014). Amyloid-beta peptide-specific DARPins as a novel class of potential therapeutics for Alzheimer disease. J. Biol. Chem. 289, 27080-27089.
0366 Yan, J., Gu, G. J., Jost, C., Hammond, M., Plückthun, A., Landegren, U., and Kamali-Moghaddam, M. (2014). A universal approach to prepare reagents for DNA-assisted protein analysis. PLoS One 9, e108061.
0365 Kiuchi, T., Ortiz-Zapater, E., Monypenny, J., Matthews, D. R., Nguyen, L. K., Barbeau, J., Coban, O., Lawler, K., Burford, B., Rolfe, D. J., de Rinaldis, E., Dafou, D., Simpson, M. A., Woodman, N., Pinder, S., Gillett, C. E., Devauges, V., Poland, S. P., Fruhwirth, G., Marra, P., Boersma, Y. L., Plückthun, A., Gullick, W. J., Yarden, Y., Santis, G., Winn, M., Kholodenko, B. N., Martin-Fernandez, M. L., Parker, P., Tutt, A., Ameer-Beg, S. M., and Ng, T. (2014). The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility. Science Signaling 7, ra78.
0364 Scholz, O., Hansen, S., and Plückthun, A. (2014). G-quadruplexes are specifically recognized and distinguished by selected designed ankyrin repeat proteins. Nucleic Acids Res 42, 9182-9194.
0362 Jost, C., and Plückthun, A. (2014). Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs. Curr. Opin. Struct. Biol. 27, 102-112.
0361 Watson, R. P., Christen, M. T., Ewald, C., Bumbak, F., Reichen, C., Mihajlovic, M., Schmidt, E., Guntert, P., Caflisch, A., Plückthun, A., and Zerbe, O. (2014). Spontaneous self-assembly of engineered armadillo repeat protein fragments into a folded structure. Structure 22, 985-995.
0360 Schilling, J., Schöppe, J., Sauer, E., and Plückthun, A. (2014). Co-Crystallization with Conformation-Specific Designed Ankyrin Repeat Proteins Explains the Conformational Flexibility of BCL-W. J. Mol. Biol. 426, 2346-2361.
0358 Reichen, C., Hansen, S., and Plückthun, A. (2014). Modular peptide binding: From a comparison of natural binders to designed armadillo repeat proteins. J. Struct. Biol. 185, 147-162.
0357 Simon, M., Stefan, N., Borsig, L., Plückthun, A., and Zangemeister-Wittke, U. (2014). Increasing the antitumor effect of an EpCAM-targeting fusion toxin by facile click PEGylation. Mol. Cancer Ther. 13, 375-385.
0356 Schilling, J., Schoeppe, J., and Plückthun, A. (2014). From DARPins to loopDARPins: Novel loopDARPin design allows the selection of low picomolar binders in a single round of ribosome display. J. Mol. Biol. 426, 691-721.
0355 Simon, M., Frey, R., Zangemeister-Wittke, U., and Plückthun, A. (2013). Orthogonal assembly of a designed ankyrin repeat protein-cytotoxin conjugate with a clickable serum albumin module for half-life extension. Bioconjugate Chem. 24, 1955-1966.
0354 Jost, C., Schilling, J., Tamaskovic, R., Schwill, M., Honegger, A., and Plückthun, A. (2013). Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. Structure 21, 1979-1991.
0353 Gigant, B., Wang, W., Dreier, B., Jiang, Q., Pecqueur, L., Plückthun, A., Wang, C., and Knossow, M. (2013). Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat. Struct. Mol. Biol. 1001-1007.
0352 Kummer, L., Hsu, C. W., Dagliyan, O., Macnevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N. V., Hahn, K. M., and Plückthun, A. (2013). Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells. Chem. Biol. 20, 847-856.
0350 Mann, A., Friedrich, N., Krarup, A., Weber, J., Stiegeler, E., Dreier, B., Pugach, P., Robbiani, M., Riedel, T., Moehle, K., Robinson, J. A., Rusert, P., Plückthun, A., and Trkola, A. (2013). Conformation-dependent recognition of HIV gp120 by designed ankyrin repeat proteins provides access to novel HIV entry inhibitors. J. Virol. 87, 5868-5881.
0349 Epa, V. C., Dolezal, O., Doughty, L., Xiao, X., Jost, C., Plückthun, A., and Adams, T. E. (2013). Structural model for the interaction of a designed ankyrin repeat protein with the human epidermal growth factor receptor 2. PLoS One 8, e59163.
0348 Friedrich, K., Hanauer, J. R., Prüfer, S., Münch, R. C., Völker, I., Filippis, C., Jost, C., Hanschmann, K. M., Cattaneo, R., Peng, K. W., Plückthun, A., Buchholz, C. J., Cichutek, K., and Mühlebach, M. D. (2013). DARPin-targeting of measles virus: unique bispecificity, effective oncolysis, and enhanced safety. Mol. Ther. 21, 849-859.
0347 Simon, M., Stefan, N., Plückthun, A., and Zangemeister-Wittke, U. (2013). Epithelial cell adhesion molecule-targeted drug delivery for cancer therapy. Expert Opin. Drug Deliv. 10, 451-468.
0346 Dreier, B., Honegger, A., Hess, C., Nagy-Davidescu, G., Mittl, P. R., Grütter, M. G., Belousova, N., Mikheeva, G., Krasnykh, V., and Plückthun, A. (2013). Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc. Natl. Acad. Sci. U. S. A. 110, E869-877.
0343 Gu, G. J., Friedman, M., Jost, C., Johnsson, K., Kamali-Moghaddam, M., Plückthun, A., Landegren, U., and Söderberg, O. (2012). Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N. Biotechnol. 30, 144-152.
0342 Varadamsetty, G., Tremmel, D., Hansen, S., Parmeggiani, F., and Plückthun, A. (2012). Designed armadillo repeat proteins: library generation, characterization and selection of peptide binders with high specificity. J. Mol. Biol. 424, 68 - 87.
0341 Frei, A. P., Jeon, O. Y., Kilcher, S., Moest, H., Henning, L. M., Jost, C., Plückthun, A., Mercer, J., Aebersold, R., Carreira, E. M., and Wollscheid, B. (2012). Direct identification of ligand-receptor interactions on living cells and tissues. Nat. Biotechnol. 30, 997-1001.
0339 Mignot, I., Pecqueur, L., Dorléans, A., Karuppasamy, M., Ravelli, R. B., Dreier, B., Plückthun, A., Knossow, M., and Gigant, B. (2012). Design and characterization of modular scaffolds for tubulin assembly. J. Biol. Chem. 287, 31085-31094.
0338 Alfarano, P., Varadamsetty, G., Ewald, C., Parmeggiani, F., Pellarin, R., Zerbe, O., Plückthun, A., and Caflisch, A. (2012). Optimization of designed armadillo repeat proteins by molecular dynamics simulations and NMR spectroscopy. Protein Science 21, 1298-1314.
0337 Kummer, L., Parizek, P., Rube, P., Millgramm, B., Prinz, A., Mittl, P. R., Kaufholz, M., Zimmermann, B., Herberg, F. W., and Plückthun, A. (2012). Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc. Natl. Acad. Sci. U. S. A. 109, E2248-2257.
0336 Parizek, P., Kummer, L., Rube, P., Prinz, A., Herberg, F. W., and Plückthun, A. (2012). Designed ankyrin repeat proteins (DARPins) as novel isoform-specific intracellular inhibitors of c-Jun N-terminal inases. ACS Chem. Biol. 7, 1356-1366.
0334 Pecqueur, L., Duellberg, C., Dreier, B., Jiang, Q., Wang, C., Plückthun, A., Surrey, T., Gigant, B., and Knossow, M. (2012). A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc. Natl. Acad. Sci. U. S. A. 109, 12011-12016.
0332 Madhurantakam, C., Varadamsetty, G., Grütter, M. G., Plückthun, A., and Mittl, P. R. (2012). Structure-based optimization of designed Armadillo-repeat proteins. Protein Science 21, 1015-1028.
0331 Simon, M., Zangemeister-Wittke, U., and Plückthun, A. (2012). Facile double-functionalization of designed ankyrin repeat proteins using click and thiol chemistries. Bioconjug. Chem. 23, 279-286.
0329 Tamaskovic, R., Simon, M., Stefan, N., Schwill, M., and Plückthun, A. (2012). Designed Ankyrin Repeat Proteins (DARPins): from research to therapy. Methods Enzymol. 503, 101-134.
0326 Boersma, Y. L., and Plückthun, A. (2011). DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr. Opin. Biotechnol. 22, 849-857.
0325 Boersma, Y. L., Chao, G., Steiner, D., Wittrup, K. D., and Plückthun, A. (2011). Bispecific Designed Ankyrin Repeat Proteins (DARPins) Targeting Epidermal Growth Factor Receptor Inhibit A431 Cell Proliferation and Receptor Recycling. J. Biol. Chem. 286, 41273-41285.
0324 Stefan, N., Martin-Killias, P., Wyss-Stoeckle, S., Honegger, A., Zangemeister-Wittke, U., and Plückthun, A. (2011). DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. J. Mol. Biol. 413, 826-843.
0320 Münch, R. C., Mühlebach, M. D., Schaser, T., Kneissl, S., Jost, C., Plückthun, A., Cichutek, K., and Buchholz, C. J. (2011). DARPins: An Efficient Targeting Domain for Lentiviral Vectors. Mol. Ther. 19, 686-693.
0318 Martin-Killias, P., Stefan, N., Rothschild, S., Plückthun, A., and Zangemeister-Wittke, U. (2011). A novel fusion toxin derived from an EpCAM-specific designed ankyrin repeat protein has potent antitumor activity. Clin. Cancer Res. 17, 100-110.
0317 Dreier, B., Mikheeva, G., Belousova, N., Parizek, P., Boczek, E., Jelesarov, I., Forrer, P., Plückthun, A., and Krasnykh, V. (2011). Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting. J. Mol. Biol. 405, 410-426.
0315 Kramer, M. A., Wetzel, S. K., Plückthun, A., Mittl, P. R., and Grütter, M. G. (2010). Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J. Mol. Biol. 404, 381-391.
0313 Theurillat, J. P., Dreier, B., Nagy-Davidescu, G., Seifert, B., Behnke, S., Zürrer-Härdi, U., Ingold, F., Plückthun, A., and Moch, H. (2010). Designed ankyrin repeat proteins: a novel tool for testing epidermal growth factor receptor 2 expression in breast cancer. Mod. Pathol. 23, 1289-1297.
0312 Wetzel, S. K., Ewald, C., Settanni, G., Jurt, S., Plückthun, A., and Zerbe, O. (2010). Residue-resolved stability of full-consensus ankyrin repeat proteins probed by NMR. J. Mol. Biol. 402, 241-258.
0310 Brient-Litzler, E., Plückthun, A., and Bedouelle, H. (2010). Knowledge-based design of reagentless fluorescent biosensors from a designed ankyrin repeat protein. Protein Eng Des Sel 23, 229-241.
0306 Zahnd, C., Kawe, M., Stumpp, M. T., de Pasquale, C., Tamaskovic, R., Nagy-Davidescu, G., Dreier, B., Schibli, R., Binz, H. K., Waibel, R., and Plückthun, A. (2010). Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. Cancer Res. 70, 1595-1605.
0305 Plückthun, A. (2009) in Recombinant Antibodies for Immunotherapy: Alternative scaffolds: Expanding the options of antibodies (Little, M., ed), pp. 243-271, Cambridge University Press, New York
0304 Veesler, D., Dreier, B., Blangy, S., Lichière, J., Tremblay, D., Moineau, S., Spinelli, S., Tegoni, M., Plückthun, A., Campanacci, V., and Cambillau, C. (2009). Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode. J. Biol. Chem. 284, 30718-30726.
0303 Winkler, J., Martin-Killias, P., Plückthun, A., and Zangemeister-Wittke, U. (2009). EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins. Molecular Cancer Ther. 9, 2674-2683.
0302 Milovnik, P., Ferrari, D., Sarkar, C. A., and Plückthun, A. (2009). Selection and characterization of DARPins specific for the neurotensin receptor 1. Protein Eng. Des. Sel. 22, 357-366.
0301 Kawe, M., Horn, U., and Plückthun, A. (2009). Facile promoter deletion in Escherichia coli in response to leaky expression of very robust and benign proteins from common expression vectors. Microbial cell factories 8, 8. . Protein Eng Des Sel 22, 135-147.
0297 Steiner, D., Forrer, P., and Plückthun, A. (2008). Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J. Mol. Biol. 382, 1211-1227.
0295 Parmeggiani, F., Pellarin, R., Larsen, A. P., Varadamsetty, G., Stumpp, M. T., Zerbe, O., Caflisch, A., and Plückthun, A. (2008). Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. J Mol Biol 376, 1282-1304.
0294 Wetzel, S. K., Settanni, G., Kenig, M., Binz, H. K., and Plückthun, A. (2008). Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. J Mol Biol 376, 241-257.
0293 Interlandi, G., Wetzel, S. K., Settanni, G., Plückthun, A., and Caflisch, A. (2008). Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments. J Mol Biol 375, 837-854.
0292 Merz, T., Wetzel, S. K., Firbank, S., Plückthun, A., Grütter, M. G., and Mittl, P. R. (2008). Stabilizing ionic interactions in a full-consensus ankyrin repeat protein. J Mol Biol 376, 232-240.
0288 Zahnd, C., Wyler, E., Schwenk, J. M., Steiner, D., Lawrence, M. C., McKern, N. M., Pecorari, F., Ward, C. W., Joos, T. O., and Plückthun, A. (2007). A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol 369, 1015-1028.
0287 Schweizer, A., Roschitzki-Voser, H., Amstutz, P., Briand, C., Gulotti-Georgieva, M., Prenosil, E., Binz, H. K., Capitani, G., Baici, A., Plückthun, A., and Grütter, M. G. (2007). Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure 15, 625-636.
0283 Kawe, M., Forrer, P., Amstutz, P., and Plückthun, A. (2006). Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening. J Biol Chem 281, 40252-40263.
0280 Zahnd, C., Pécorari, F., Straumann, N., Wyler, E., and Plückthun, A. (2006). Selection and characterization of Her2 binding-designed ankyrin repeat proteins. J Biol Chem 281, 35167-35175.
0279 Yu, H., Kohl, A., Binz, H. K., Plückthun, A., Grütter, M. G., and van Gunsteren, W. F. (2006). Molecular dynamics study of the stabilities of consensus designed ankyrin repeat proteins. Proteins 65, 285-295.
0278 Binz, H. K., Kohl, A., Plückthun, A., and Grütter, M. G. (2006). Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability. Proteins 65, 280-284.
0276 Steiner, D., Forrer, P., Stumpp, M. T., and Plückthun, A. (2006). Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat Biotechnol 24, 823-831.
0273 Amstutz, P., Koch, H., Binz, H. K., Deuber, S. A., and Plückthun, A. (2006). Rapid selection of specific MAP kinase-binders from designed ankyrin repeat protein libraries. Protein Eng. Des. Sel. 19, 219-229.
0270 Li, L., Wetzel, S., Plückthun, A., and Fernandez, J. M. (2006). Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy. Biophys J 90, L30-32.
0263 Schimmele, B., and Plückthun, A. (2005). Identification of a functional epitope of the Nogo receptor by a combinatorial approach using ribosome display. J Mol Biol 352, 229-241.
0262 Kohl, A., Amstutz, P., Parizek, P., Binz, H. K., Briand, C., Capitani, G., Forrer, P., Plückthun, A., and Grütter, M. G. (2005). Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure (Camb) 13, 1131-1141.
0260 Amstutz, P., Binz, H. K., Parizek, P., Stumpp, M. T., Kohl, A., Grütter, M. G., Forrer, P., and Plückthun, A. (2005). Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem 280, 24715-24722.
0252 Devi, V. S., Binz, H. K., Stumpp, M. T., Plückthun, A., Bosshard, H. R., and Jelesarov, I. (2004). Folding of a designed simple ankyrin repeat protein. Protein Sci. 13, 2864-2870.
0249 Binz, H. K., Amstutz, P., Kohl, A., Stumpp, M. T., Briand, C., Forrer, P., Grütter, M. G., and Plückthun, A. (2004). High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol 22, 575-582.
0245 Forrer, P., Binz, H. K., Stumpp, M. T., and Plückthun, A. (2004). Consensus design of repeat proteins. ChemBioChem 5, 183-189.
0239 Binz, H. K., Stumpp, M. T., Forrer, P., Amstutz, P., and Plückthun, A. (2003). Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol 332, 489-503.
0238 Stumpp, M. T., Forrer, P., Binz, H. K., and Plückthun, A. (2003). Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. J Mol Biol 332, 471-487.
0233 Forrer, P., Stumpp, M. T., Binz, H. K., and Plückthun, A. (2003). A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett 539, 2-6.
0231 Kohl, A., Binz, H. K., Forrer, P., Stumpp, M. T., Plückthun, A., and Grütter, M. G. (2003). Designed to be stable: Crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A 100, 1700-1705.