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Department of Biochemistry

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Publications of the Gehring Group

Zhou, Y., Yu, J., Lei, X., Niu, Q., Zhang, Y., Liu, H., Christen, P., Gehring , H. & Wu, F. High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway. Chem Comm (Camb.) 49, 11782-11784 (2013).

Fernandez, FJ., de Vries, D., Pena-Soler, E., Coll, M., Christen, P., Gehring, H. & Vega, MC. Structure and mechanism of a cysteine sulfinate desulfinase engineered on the aspartate aminotransferase scaffold. Biochim Biophys Acta. 1824, 339-349 (2012).

Leemann-Zakaryan, R., Pahlich, S., Grossenbacher, D. & Gehring, H. Tyrosine phosphorylation in the C-terminal nuclear localization and retention signal (C-NLS) of the EWS protein. Sarcoma 2011, ID 218483 (2011).

Wu, F., Christen, P. & Gehring, H. A novel approach to inhibit intracellular vitamin B6-dependent enzymes: proof of principle with human and plasmodium ornithine decarboxylase and human histidine decarboxylase. FASEB J. 25, 2109-2122 (2011).

Müller, IB., Wu, F., Bergmann, B., Knöckel, J., Walter, RD., Gehring, H. & Wrenger, C. Poisoning pyridoxal 5-phosphate-dependent enzymes: a new strategy to target the malaria parasite plasmodium falciparumPLoS ONE. 4(2):e4406 (2009).

Leemann-Zakaryan, R., Pahlich, S., Sedda, M., Quero, L., Grossenbacher, D. & Gehring, H. Dynamic subcellular localization of Ewing Sarcoma proto-oncoprotein and its association with and stabilization of microtubules. J. Mol. Biol. 386, 1-13 (2009).

Pahlich, S., Quero, L., Roschitzki, B., Leemann-Zakaryan, RP. & Gehring, H. Analysis of Ewing Sarcoma (EWS)-binding proteins: interaction with hnRNP M, U and RNA-helicases p68/72 with protein-RNA complexes. J. Proteome Res. 8, 4455-4465 (2009).  

Wu, F. & Gehring, H. Structural requirements for novel coenzyme-substrate derivatives to inhibit intracellular ornithine decarboxylase and cell proliferation.  FASEB J. 23, 565-574 (2009).

Curioni-Fontecedro, A., Knights, AJ., Tinguely, M., Nuber, N., Schneider, C., Thomson, CW., von Boehmer, L., Bossart, W., Pahlich, S.,  Gehring, H., Moch, H., Renner, C., Knuth, A.  & Zippelius, A. MAGE -C1/CT7 is the dominant cancer-testis antigen targeted by humoral immune responses in patients with multiple myeloma. Leukemia 22, 1646-1648 (2008).

Pahlich, S. Zakaryan, RP. & Gehring, H. Identification of proteins interacting with protein arginine methyltransferase 8: The Ewing sarcoma (EWS) protein binds independent of its methylation state. Proteins 72, 1125-1137 (2008).

Wu, F., Yu, J. & Gehring, H. Inhibitory and structural studies of novel coenzyme-substrate analogs of human histidine decarboxylase. FASEB J.  22, 890-897 (2008).

Wu, F., Grossenbacher, D. & Gehring, H. New transition state–based inhibitor for human ornithine decarboxylase inhibits growth of tumor cells. Mol. Cancer Ther. 6, 1–9 (2007).

Pahlich, S., Zakaryan, R.P. & Gehring, H. (2006) Protein arginine methylation: Cellular functions and methods of analysis, Biochimica et Biophysica Acta 1764, 1890-1903 (2006). 

Zakaryan, R.P. & Gehring, H. Identification and Characterization of the Nuclear Localization/Retention Signal in the EWS Proto-oncoprotein. J. Mol. Biol. 363, 27-38 (2006).

Pahlich, S., Bschir, K., Chiavi, C., Belyanskaya, L. & Gehring, H. Different methylation characteristics of protein arginine methyltransferase 1 and 3 toward the Ewing sarcoma protein and a peptide. Proteins-Structure Function and Bioinformatics 61, 164-175 (2005).

Fernandez, F. J., Vega, M. C., Lehmann, F., Sandmeier, E., Gehring, H., Christen, P. & Wilmanns, M. Structural studies of the catalytic reaction pathway of a hyperthermophilic histidinol-phosphate aminotransferase. Journal of Biological Chemistry 279, 21478-21488 (2004).

Belyanskaya, L. L., Delattre, O. & Gehring, H. Expression and subcellular localization of Ewing sarcoma (EWS) protein is affected by the methylation process. Experimental Cell Research 288, 374-381 (2003).

Gygi, D., Zumstein, P., Grossenbacher, D., Altwegg, L., Luscher, T. F. & Gehring, H. Human connective tissue growth factor expressed in Escherichia coli is a non-mitogenic inhibitor of apoptosis. Biochemical and Biophysical Research Communications 311, 685-690 (2003).

Minder, D., Boni, J., Schupbach, J. & Gehring, H. Immunophilins and HIV-1 infection. Archives of Virology 147, 1531-1542 (2002).

Belyanskaya, L. L., Gehrig, P. M. & Gehring, H. Exposure on cell surface and extensive arginine methylation of ewing sarcoma (EWS) protein. Journal of Biological Chemistry 276, 18681-18687 (2001).

Kasper, P., Christen, P. & Gehring, H. Empirical calculation of the relative free energies of peptide binding to the molecular chaperone DnaK. Proteins-Structure Function and Genetics 40, 185-192 (2000).

Endrich, M. M., Gehrig, P. & Gehring, H. Maturation-induced conformational changes of HIV-1 capsid protein and identification of two high affinity sites for cyclophilins in the C-terminal domain. Journal of Biological Chemistry 274, 5326-5332 (1999).

Graber, R., Kasper, P., Malashkevich, V. N., Strop, P., Gehring, H., Jansonius, J. N. & Christen, P. Conversion of aspartate aminotransferase into an L-aspartate beta-decarboxylase by a triple active-site mutation. J. Biol. Chem. 274, 31203-31208 (1999).    
    
Mouratou, B., Kasper, P., Gehring, H. & Christen, P. Conversion of tyrosine phenol-lyase to dicarboxylic amino acid beta-lyase, an enzyme not found in nature. J. Biol. Chem. 274, 1320-1325 (1999).    
    
Endrich, M. M. & Gehring, H. The V3 loop of human immunodeficiency virus type-1 envelope protein is a high-affinity ligand for immunophilins present in human blood. Eur. J. Biochem. 252, 441-446 (1998).    
    
Christen, P., Kasper, P., Gehring, H. & Sterk, M. Stereochemical constraint in the evolution of pyridoxal-5'-phosphate-dependent enzymes. A hypothesis. FEBS Lett. 389, 12-14 (1996).    
    
Endrich, M. M., Grossenbacher, D., Geistlich, A. & Gehring, H. Apoptosis-induced concomitant release of cytosolic proteins and factors which prevent cell death. Biol. Cell 88, 15-22 (1996).    
    
Kasper, P., Sterk, M., Christen, P. & Gehring, H. Molecular-dynamics simulation of domain movements in aspartate aminotransferase. Eur. J. Biochem. 240, 751-755 (1996).    
    
Graber, R., Kasper, P., Malashkevich, V. N., Sandmeier, E., Berger, P., Gehring, H., Jansonius, J. N. & Christen, P. Changing the Reaction Specificity of a Pyridoxal-5'-Phosphate-Dependent Enzyme. Eur. J. Biochem. 232, 686-690 (1995).    
    
Malashkevich, V. N., Jager, J., Ziak, M., Sauder, U., Gehring, H., Christen, P. & Jansonius, J. N. Structural Basis for the Catalytic Activity of Aspartate-Aminotransferase K258h Lacking the Pyridoxal 5'-Phosphate-Binding Lysine Residue. Biochemistry 34, 405-414 (1995).    
    
Pan, P., Jakob, C. A., Sandmeier, E., Christen, P. & Gehring, H. Modulation of the Activity of Mitochondrial Aspartate-Aminotransferase H352c by the Redox State of the Engineered Interdomain Disulfide Bond. J. Biol. Chem. 269, 25432-25436 (1994).    
    
Pan, P., Jaussi, R., Gehring, H., Giannattasio, S. & Christen, P. Shift in Ph-Rate Profile and Enhanced Discrimination between Dicarboxylic and Aromatic Substrates in Mitochondrial Aspartate-Aminotransferase Y70h. Biochemistry 33, 2757-2760 (1994).    
    
Schmid, D., Baici, A., Gehring, H. & Christen, P. Kinetics of Molecular Chaperone Action. Science 263, 971-973 (1994).    
    
Sterk, M., Hauser, H., Marsh, D. & Gehring, H. Probing Conformational States of Spin-Labeled Aspartate-Aminotransferase by Esr. Eur. J. Biochem. 219, 993-1000 (1994)

Geistlich, A. & Gehring, H. Cdgf (Chicken-Embryo Fibroblast-Derived Growth-Factor) Is Mitogenically Related to Tgf-Beta and Modulates Pdgf, Bfgf, and Igf-I Action on Sparse Nih/3t3 Cells. Exp. Cell Res. 204, 329-335 (1993).

Hartmann, C. M., Gehring, H. & Christen, P. The Mature Form of Imported Mitochondrial Proteins Undergoes Conformational-Changes Upon Binding to Isolated-Mitochondria. Eur. J. Biochem. 218, 905-910 (1993).

Ziak, M., Jager, J., Malashkevich, V. N., Gehring, H., Jaussi, R., Jansonius, J. N. & Christen, P. Mutant Aspartate-Aminotransferase (K258h) without Pyridoxal-5'-Phosphate-Binding Lysine Residue - Structural and Catalytic Properties. Eur. J. Biochem. 211, 475-484 (1993).

Geistlich, A. & Gehring, H. Isolation and Characterization of a Novel Type of Growth-Factor Derived from Serum-Free Conditioned Medium of Chicken-Embryo Fibroblasts. Eur. J. Biochem. 207, 147-153 (1992).

Gehring, H. & Schroder, A. Thymidine Secretion by Cultured Chicken-Embryo Fibroblasts and Nih/3t3 Cells - Quantification and Time Course. Biochem. Biophys. Res. Commun. 177, 259-264 (1991).

Sterk, M. & Gehring, H. Spectroscopic Characterization of True Enzyme-Substrate Intermediates of Aspartate-Aminotransferase Trapped at Subzero Temperatures. Eur. J. Biochem. 201, 703-707 (1991).

Ziak, M., Jaussi, R., Gehring, H. & Christen, P. Aspartate-Aminotransferase with the Pyridoxal-5'-Phosphate-Binding Lysine Residue Replaced by Histidine Retains Partial Catalytic Competence. Eur. J. Biochem. 187, 329-333 (1990).    
    
Sharma, C. P. & Gehring, H. Effect of Vitamin-B6 on the Synthesis and Degradation of Aspartate-Aminotransferase in Chicken-Embryo Fibroblasts. J. Biol. Chem. 262, 16503-16508 (1987).    
    
Sharma, C. P. & Gehring, H. A Low-Molecular-Weight Growth Inhibitor Secreted in Cultures of Chicken-Embryo Fibroblasts. Experientia 43, 638-639 (1987).    
    
Tobler, H. P., Gehring, H. & Christen, P. Stereospecific Labilization of the C-4' Pro-S Hydrogen of Pyridoxamine 5'-Phosphate in Aspartate-Aminotransferase - Activators and Inhibitors. J. Biol. Chem. 262, 8985-8989 (1987).    
    
Gehring, H. Cryoenzymological Study of Aspartate-Aminotransferase - Detection of Intermediates by Monitoring Single Turnovers with a True Substrate. Eur. J. Biochem. 159, 291-296 (1986).    
    
Sharma, C. P. & Gehring, H. A Low-Molecular-Weight Growth Inhibitor Secreted in Cultures of Chicken-Embryo Fibroblasts. Biochem. Biophys. Res. Commun. 139, 1243-1249 (1986).    
    
Sharma, C. P. & Gehring, H. The Precursor of Mitochondrial Aspartate-Aminotransferase Is Translocated into Mitochondria as Apoprotein. J. Biol. Chem. 261, 1146-1149 (1986).    
    
Tobler, H. P., Christen, P. & Gehring, H. Stereospecific Labilization of the C4'-Pro-S Hydrogen of Pyridoxamine-5'-Phosphate by Aspartate-Aminotransferase. Experientia 42, 683-683 (1986).    
    
Tobler, H. P., Christen, P. & Gehring, H. Stereospecific Labilization of the C-4' Pro-S Hydrogen of Pyridoxamine 5'-Phosphate in Aspartate-Aminotransferase. J. Biol. Chem. 261, 7105-7108 (1986).    
    
Christen, P., Gehring, H., Sandmeier, E., Kirsten, H., Pfister, K. & Jansonius, J. N. Correlation of Structure and Catalysis by Aspartate-Aminotransferase Isoenzymes. Biochem. Soc. Transac. 12, 427-430 (1984).    
    
Gehring, H. Transfer of C-Alpha-Hydrogen of Glutamate to Coenzyme of Aspartate-Aminotransferase during Transamination Reaction. Biochemistry 23, 6335-6340 (1984).

Gehring, H. Cryoenzymological Investigation of the Catalytic Mechanism of Aspartate-Aminotransferase (Aspat). Experientia 40, 607-607 (1984).

Jansonius, J. N., Eichele, G., Ford, G. C., Kirsch, J. F., Picot, D., Thaller, C., Vincent, M. G., Gehring, H. & Christen, P. 3 Dimensional Structure of Mitochondrial Aspartate-Aminotransferase and Some Functional-Derivatives - Implications for Its Mode of Action. Biochem. Soc. Transac. 12, 424-427 (1984).

Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., Gehring, H. & Christen, P. Mechanism of Action of Aspartate-Aminotransferase Proposed on the Basis of Its Spatial Structure. J. Mol. Biol. 174, 497-525 (1984).

Sharma, P. & Gehring, H. Effect of Pyridoxal (Pl) Deficiency on Cytosolic (C) and Mitochondrial (M) Aspartate-Aminotransferase (Aspat) in Chicken-Embryo Fibroblasts. Experientia 40, 636-637 (1984).

Tobler, H. P., Gehring, H. & Christen, P. Apoenzyme-Catalyzed Model Reactions for Enzymic Transamination. Experientia 40, 614-615 (1984).

Gehring, H. & Christen, P. Isolation of Sulfhydryl Peptides Alkylated with N-Ethylmaleimide by Diagonal Electrophoresis. Meth. Enzymol. 91, 392-396 (1983).

Heller, M. & Gehring, H. Interaction of Omega-Substituted Analogs of Dicarboxylic Amino-Acids with Aspartate-Aminotransferase (Aspat). Experientia 39, 648-648 (1983).

Kirsten, H., Gehring, H. & Christen, P. Crystalline Aspartate-Aminotransferase - Lattice-Induced Functional Asymmetry of the 2 Subunits. Proc. Natl. Acad. Sci. USA 80, 1807-1810 (1983).

Meer, L. & Gehring, H. Limited Proteolytic Cleavage of Aspartate-Aminotransferase Isoenzymes (Aat). Experientia 38, 728-729 (1982).

Sonderegger, P., Jaussi, R., Christen, P. & Gehring, H. Biosynthesis of Aspartate Aminotransferases - Both the Higher Molecular-Weight Precursor of Mitochondrial Aspartate-Aminotransferase and the Cytosolic Isoenzyme Are Synthesized on Free Polysomes. J. Biol. Chem. 257, 3339-3345 (1982).

Sonderegger, P., Jaussi, R., Gehring, H., Brunschweiler, K. & Christen, P. Peptide-Mapping of Protein Bands from Polyacrylamide-Gel Electrophoresis by Chemical Cleavage in Gel Pieces and Re-Electrophoresis. Anal. Biochem. 122, 298-301 (1982).

Sonderegger, P., Jaussi, R., Gehring, H., Brunschweiler, K. & Christen, P. Direct Peptide-Mapping of Protein Bands from Polyacrylamide-Gel Electrophoresis by Chemical Cleavage in Gel Pieces and Re-Electrophoresis. Experientia 38, 732-732 (1982).

Jansonius, J. N., Eichele, G., Ford, G. C., Kirsch, J. F., Picot, D., Thaller, C., Vincent, M. G., Christen, P., Gehring, H. & Kirsten, H. Conformational-Changes during Catalysis by Mitochondrial Aspartate-Aminotransferase. Federation Proceedings 40, 1757-1757 (1981).

Kirsten, H., Gehring, H. & Christen, P. Catalytic Properties of Crystalline Mitochondrial Aspartate-Aminotransferase, Lattice-Induced Functional Asymmetry of the 2 Subunits. Experientia 37, 627-627 (1981).Gehring, H. A New Diagonal Procedure for Isolating Sulfhydryl Peptides Alkylated with N-Ethylmaleimide. Experientia 36, 721-721 (1980).

Gehring, H. & Christen, P. A Diagonal Procedure for Isolating Sulfhydryl Peptides Alkylated with N-Ethylmaleimide. Anal. Biochem. 107, 358-361 (1980).

Thompson, J. S., Gehring, H. & Vallee, B. L. Structure and Function of Carboxypeptidase-a-Alpha in Supercooled Water. Proc. Natl. Acad. Sci. USA 77, 132-136 (1980).    
    
Gehring, H. & Christen, P. Syncatalytic Conformational-Changes in Mitochondrial Aspartate Aminotransferases - Evidence from Modification and Demodification of Cys 166 in Enzyme from Chicken and Pig. J. Biol. Chem. 253, 3158-3163 (1978).    
    
Gehring, H., Christen, P., Eichele, G., Glor, M., Jansonius, J. N., Reimer, A. S., Smit, J. D. G. & Thaller, C. Isolation, Crystallization and Preliminary Crystallographic Data of Aspartate-Aminotransferase from Chicken Heart-Mitochondria. J. Mol. Biol. 115, 97-101 (1977).    
    
Gehring, H., Rando, R. R. & Christen, P. Active-Site Labeling of Aspartate Aminotransferases by Beta,Gamma-Unsaturated Amino-Acid Vinylglycine. Biochemistry 16, 4832-4836 (1977).

Sonderegger, P., Gehring, H. & Christen, P. Interspecies Comparison of Cytosolic and Mitochondrial Aspartate Aminotransferases - Evidence for a More Conservative Evolution of Mitochondrial Isoenzyme. J. Biol. Chem. 252, 609-612 (1977).    
    
Gehring, H. & Christen, P. Release of 5-Thio (2-Nitrobenzoate) from Mixed Disulfide Derivative of Mitochondrial Aspartate-Aminotransferase - Probe for Syncatalytic Conformational-Changes. Experientia 32, 768-768 (1976).    
    
Gehring, H. & Christen, P. Syncatalytic Sulfhydryl Group Modification in Mitochondrial Aspartate-Aminotransferase from Chicken and Pig Heart. Experientia 31, 719-719 (1975).    
    
Gehring, H. & Christen, P. Syncatalytic Sulfhydryl Group Modification in Mitochondrial Aspartate-Aminotransferase from Chicken and Pig Heart. Biochem. Biophys. Res. Commun. 63, 441-447 (1975).

Gehring, H., Wilson, K. J. & Christen, P. Conspicuous Degree of Homology between Mitochondrial Aspartate Aminotransferases from Chicken and Pig Heart. Biochem. Biophys. Res. Commun. 67, 73-78 (1975).