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Department of Biochemistry

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Publications of the Medalia Group (in alphabetical order)

2017
de Leeuw, R., Gruenbaum, Y. & Medalia, O. Nuclear lamins: thin filaments with major functions. Trends Cell Biol. [Epub ahead of print] (2017). PMID: 28893461.

Malka-Gibor, E., Kornreich, M., Laser-Azogui, A., Doron, O., Zingerman-Koladko, I., Harapin, J., Medalia, O. & Beck, R. Phosphorylation-Induced Mechanical Regulation of Intrinsically Disordered Neurofilament Proteins. Biophys. J. 112, 892-900 (2017). PMID: 28297648.

Turgay, Y. & Medalia, O. The structure of lamin filaments in somatic cells as revealed by cryo-electron tomography. Nucleus [Epub ahead of print] (2017). PMID: 28635493.

Turgay, Y., Eibauer, M., Goldman, AE., Shimi, T., Khayat, M., Ben-Harush, K., Dubrovsky-Gaupp, A., Sapra, KT., Goldman, RD. & Medalia, O. The molecular architecture of lamins in somatic cells. Nature 543, 261-264 (2017). PMID: 28241138.

2016
Dubey, GP., Malli Mohan, GB., Dubrovsky, A., Amen, T., Tsipshtein, S., Rouvinski, A., Rosenberg, A., Kaganovich, D., Sherman, E., Medalia, O. & Ben-Yehuda, S. Architecture and Characteristics of Bacterial Nanotubes. Dev. Cell 36, 453-61 (2016). PMID: 26906740.

Irobalieva, RN., Martins, B. & Medalia, O. Cellular structural biology as revealed by cryo-electron tomography. J. Cell. Sci. 129, 469-76 (2016). PMID: 26787742.

Sorrentino, S., Studt, JD., Bokstad Horev, M., Medalia, O. & Sapra, KT. Toward correlating structure and mechanics of platelets. Cell Adh Migr 10, 568-575 (2016). PMID: 27104281.

Tamir, A., Sorrentino, S., Motahedeh, S., Shai, E., Dubrovsky, A., Dahan, I., Eibauer, M., Studt, JD., Tanuj Sapra, K., Varon, D. & Medalia, O. The macromolecular architecture of platelet-derived microparticles. J. Struct. Biol. (J. Struct. Biol. 193, 181-7 (2016). PMID: 26767592.

Zingerman-Koladko, I., Khayat, M., Harapin, J., Shoseyov, O., Gruenbaum, Y., Salman, A., Medalia, O. & Ben-Harush, K. The assembly of C. elegans lamins into macroscopic fibers. J Mech Behav Biomed Mater 63, 35-43 (2016). PMID: 27341289.

Zuela, N., Zwerger, M., Levin, T., Medalia, O. & Gruenbaum, Y. Impaired mechanical response of an EDMD mutation leads to motility phenotypes that are repaired by loss of prenylation. J. Cell. Sci. 129, 1781-91 (2016). PMID: 27034135.

Zwerger, M., Eibauer, M. & Medalia, O. Insights into the gate and scaffold of the nuclear pore complex. Nucleus  7, 1-7 (2016). PMID: 26902931.

2015
Dubrovsky, A., Sorrentino, S., Harapin, J., Sapra, KT. & Medalia, O. Developments in cryo-electron tomography for in situ structural analysis. Arch.  Arch. Biochem. Biophys. 581, 78-85 (2015). PMID: 25921875.

Eibauer, M., Pellanda, M., Turgay, Y., Dubrovsky, A., Wild, A. & Medalia, O. Structure and gating of the nuclear pore complex. Nat Commun 6, 7532 (2015). PMID: 26112706.

Gruenbaum, Y. & Medalia, O. Lamins: the structure and protein complexes. Curr. Opin. Cell Biol. 32, 7-12 (2015). PMID: 25460776.

Harapin, J., Börmel, M., Sapra, KT., Brunner, D., Kaech, A. & Medalia, O. Structural analysis of multicellular organisms with cryo-electron tomography. Nat. Methods 12, 634-6 (2015). PMID: 25961413.

Sorrentino, S., Studt, JD., Medalia, O. & Tanuj Sapra, K. Roll, adhere, spread and contract: structural mechanics of platelet function. Eur. J. Cell Biol. 94, 129-38 (2015). PMID: 25655000.

Zwerger, M., Roschitzki-Voser, H., Zbinden, R., Denais, C., Herrmann, H., Lammerding, J., Grütter, MG. & Medalia, O. Altering lamina assembly reveals lamina-dependent and -independent functions for A-type lamins. J. Cell. Sci. 128, 3607-20 (2015). PMID: 26275827.

2013
Elad, N, Volberg, T., Patla, I., Hirschfeld-Warneken, V., Grashoff, C., Spatz, JP., Fässler, R., Geiger, B. & Medalia, O. The role of integrin-linked kinase in the molecular architecture of focal adhesions. J Cell Sci. 126, 4099-4107 (2013).

Harapin, J., Eibauer, M. & Medalia, O. Structural analysis of supramolecular assemblies by cryo-electron tomography. Structure 21, 1522-30 (2013).

Lavelin, I., Wolfenson, H., Patla, I., Henis, YI., Medalia, O., Volberg, T., Livne, A., Kam, Z. & Geiger, B. Differential effect of actomyosin relaxation on the dynamic properties of focal adhesion proteins. PLoS ONE 8, e73549 (2013).

Sandler, I., Medalia, O. & Aharoni, A. Experimental analysis of co-evolution within protein complexes: the yeast exosome as a model. Proteins 81, 1997-2006 (2013).

Zwerger, M. & Medalia, O. From lamins to lamina: a structural perspective. Histochem Cell Biol. 140, 3-12 (2013).

2012
Bank, EM., Ben-Harush, K.,  Feinstein, N., Medalia, O. & Gruenbaum, Y. Structural and physiological phenotypes of disease-linked lamin mutations in C. elegans. J Struct. Biol. 177, 106-112 (2012).

Bokstad, M., Sabanay, H., Dahan, I., Geiger, B. & Medalia, O. Reconstructing adhesion structures in tissues by cryo-electron tomography of vitrified frozen sections. J. Struct. Biol. 178, 76-83 (2012).

Fridmann, K., Mader, A., Zwerger, M., Elia, N. & Medalia, O. Advances in tomography: probing the molecular architecture of cells. Nat Rev. Mol Cell Biol. 13, 736-742 (2012).

Grossman, E., Dahan, I., Stick, R., Goldberg, MW., Gruenbaum, Y. & Medalia, O. Filament assembly of ectopically expressed Caenorhabditis elegans lamin within Xenopus oocytes. J. Struct. Biol. 177, 113-118 (2012).

Grossmann, E., Medalia, O. & Zwerger, M. Functional architecture of the nuclear pore complex. Annu Rev Biophys. 41, 557-584 (2012).

Henderson, R, Sali, A, Baker, ML, Carragher B, Devkota B, Downing KH, Egelman EH, Feng Z, Frank J, Grigorieff N, Jiang W, Ludtke SJ, Medalia O, Penczek PA, Rosenthal PB, Rossmann MG, Schmid MF, Schröder GF, Steven AC, Stokes DL, Westbrook JD, Wriggers W, Yang H, Young J, Berman HM, Chiu W, Kleywegt GJ & Lawson CL. Outcome of the first electron microscopy validation task force meeting. Structure 20, 205-2014 (2012).

Maimon, T., Elad, N., Dahan, I. & Medalia, O. The human nuclear pore complex as revealed by cryo-electron tomography. Structure 20, 998-1006 (2012).

Medalia, O., & Frangakis, A. Special issue on electron tomography. J Struct Biol. 178, 75 (2012).

Rigort, A., Günther, D., Hegert, R., Baum, D., Weber, B., Prohaska, S., Medalia, O., Baumeister, W. & Hege, HC. Automated segmentation of electron tomograms for a quantitative description of actin filament networks. J Struct Biol. 177, 135-144 (2012).

Zwerger, M. & Medalia, O. Unravelling the lamina network. Nat. Rev. Mol. Cell Biol. 13, 140 (2012).

2011
Bank, EM., Ben-Harush, K., Wiesel-Motiuk, N., Barkan, R., Feinstein, N., Lotan, O., Medalia, O. & Gruenbaum, Y. A laminopathic mutation disrupting lamin filament assembly causes disease-like phenotypes in C. elegans. Mol Biol Cell. 22, 2716-2728 (2011).

Elad, N., Abramovitch, S., Sabanay, H. & Medalia, O. Microtubule organization in the final stages of cytokinesis as revealed by cryo-electron tomography. J Cell Sci. 124, 207-215 (2011). 

Tamir, A., Elad, N. & Medalia, O. Assembly and breakdown of microtubules within the midbody. Commun Integr. Biol. 5, 552-553 (2011). 

Yahav, T., Maimon, T., Grossman, E., Dahan, I. & Medalia, O. Cryo-electron tomography: gaining insight into cellular processes by strcutural approaches. Curr Opin Struct Biol. 21, 670-677 (2011).

2010
Ben-Harush, K., Maimon T., Villa, E. & Medalia, O. Visualizing cellular processes at the molecular level by cryo-electron tomography. J. Cell Sci123, 7-12 (2010). 

Frenkiel-Krispin, D., Mako, B., Aebi, U. &  Medalia, O. Structural analysis of a metazoan nuclear pore complex reveals fused concentric rings architectureJ. Mol. Biol. 395, 578-586 (2010). 

Mader, A., Ekad, N. & Medalia, O. Cryoelectron tomography of eukaryotic cells. Methods Enzymol483, 245-265 (2010). 

Maimon, T. & Medalia, O. Perspective on the metazoan nuclear pore complex. Nucleus 1, 383-386 (2010). 

Medalia, O. & Geiger, B. Frontiers of microscopy-based research into cell-matrix adhesions. Curr Opin Cell Biol. 22, 659-668 (2010).

Patla, I., Volberg, T., Elad, N., Hirschfeld-Warneken,V., Grashoff, C., Fässler, R., Spatz, JP., Geiger, B. & Medalia, O. Dissecting molecular architecture of integrin adhesion sites by cryo-electron tomography. Nat Cell Biol. 12, 909-915 (2010).

2009
Ben-Harush, K., Wiesel, N., Frenkiel-Krispin, D., Herrmann, H., Aebi, U., Gruenbaum, Y. & Medalia, O. Dissecting the supramolecular organization of the nuclear lamin filaments and paracrystalline fibers. J. Mol. Biol. 386, 1392-13402 (2009).

Böttcher, R., Wiesner, S., Braun, A., Wimmer, R., Berna, A., Elad, E., Medalia, O., Pfeifer, A., Aszódi, A., Costell, M. &  Fässler, R.  Profilin 1 is required for midbody separation during late cytokinesis of chondrocytes.  EMBO J. 28, 1157-1169 (2009).

Elad N., Maimon T., Frenkiel-Krispin, D., Lim R.Y.H. & Medalia, O. Structural analysis of the nuclear pore complex by integrated approachesCurr Opin Struct Biol. 19, 226-232 (2009).

Konorty, M., Kahana, N., Brumfeld, V., Vermiglio, A., Medalia, O. & Minsky, A. Photosynthetic system in Blastochloris viridis revisited. Biochem.  48, 4753-4761 (2009).

Lieber, A., Kushmaro, A., Minsky A. & Medalia, O. (2009) Chromatin organization and radio-resistance in the bacterium Gemmata obscuriglobus.  J. Bacteriol. 191, 1439-1445 (2009). 

Medalia, N.,  Beer, A., Zwickl, P., Mihalache, O., Beck, M., Medalia, O. & Navon, A. Architecture and molecular mechanism of PAN, the archaeal proteasome regulatory ATPase.  J Biol. Chem. 284, 22952-22960 (2009).

Taimen, P., Pfleghaar, K., Shimi, T., Möller, D., Ben-Harush, K., Erdos, MR., Adam, SA., Herrmann, H., Medalia, O., Collins, F., Goldman, AE. & Goldman, R.  A progeria mutation reveals functions for lamin A in nuclear assembly, architecture and chromosome organization. Proc Natl. Acad. Sci. U.S.A106, 20788-20793 (2009).

2008
Beck, M. & Medalia, O. Structural and functional insights into nucleocytoplasmic transport. Histol. Histopathol. 23, 1025-1033 (2008).

Gruska, M., Medalia, O., Baumeister, W. &  Leis, A. Electron tomography of vitreous sections from cultured mammalian cells.  J. Struct. Biol. 161,  383-392 (2008).       

Konorty, M., Kahana, N., Linaroudis, A., Minsky, A. &  Medalia, O. Structural analysis of photosynthetic membranes by cryo-electron tomography of intact Rhodopseudomonas viridis cells. J. Struct. Biol161, 393-400 (2008).

Marko, M., Ellisman, M. & Medalia, O. The 4th international conference on electron tomography. Editorial.  J. Struct. Biol. 161, 219 (2008).

Wiesel, N.,  Mattout, A., Melcer, S., Melamed-Book, N., Herrmann, H., Medalia, O., Aebi, U. & Gruenbaum, Y.  Laminopathic mutations interfere with the assembly, localization and dynamics of nuclear lamins. Proc Natl. Acad. Sci. U.S.A105, 180-185 (2008).

2007
Abu-Qarn, M., Yurist, S., Giordano,S., Trauner, A., Morris, H.R., Hitchen, P., Medalia, O., Dell, A. & Eichler, J.  Haloferax volcanii AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer. J. Mol. Bio 374, 1224-1236 (2007).

Beck, M., Lucic, V., Förster, F.,  Baumeister, W. & Medalia, O. Snapshots of nuclear pore complexes in action captured by cryoelectron tomography. Nature  449, 611-615  (2007).   

Medalia, O., Beck, M., Ecke, M., Weber, I., Neujahr, R., Baumeister,W. Gerisch, G.  Organization of actin networks in intact filopodia. Curr. Biol17, 79-84 (2007).

2006
Medalia, N., Sharon, M., Martinez-Arias, R., Mihalache, O,, Robinson, CV, Medalia, O& Zwickl, P. Functional and structural characterization of the Methanosarcina mazei proteasome and PAN complexes. J. Struct. Biol. 156, 84-92 (2006).            

2005
Förster, F., Medalia, O., Zauberman, N., Baumeister, W., & Fass, D. Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proc Natl. Acad. Sci. U.S.A. 102, 4729-4734 (2005).  

2004
Beck, M., Forster, F., Ecke, M., Plitzko, JM., Melchior, F., Gerisch, G., Baumeister, W. &  Medalia, O.  Nuclear pore complex structure and dynamics revealed by cryoelectron tomography.  Science 306, 1387-90 (2004). 

Kürner, J., Medalia O.,  Linaroudis, AA. & Baumeister, W. New insights into the structural organization of eukaryotic and prokaryotic cytoskeletons using cryo-electron tomography. Exp. Cell Res. 301, 38-42 (2004).

2003
Uhle, U., Medalia, O., Dumdey, R., Henklein, P., Bech-Otschir, D., Berndt, C., Berse, M., Sperling, J. &  Dubiel, W. Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome.  EMBO J. 22, 1302-131 (2003).

2002
Bretschneider, T., Jonkman, J., Köhler, J., Medalia, O., Weber, I., Stelzer, E., Baumeister, W. & Gerisch, G.  Dynamic organization of the actin system in the motile cells of Dictyostelium. J Muscle Res Cell Motil23, 639-649 (2002) .

Grünewald, K., Medalia, O., Gross, A., Steven, A.C. & Baumeister, W. Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding. Biophys. Chem100, 577-591 (2002) .

Medalia, O., Weber, I., Frangakis, A.S., Nicastro, D., Gerisch, G. & Baumeister, W. Macromolecular architecture in eukaryotic cells visualized by cryo-electron tomography.  Science 298, 1209-1213 (2002).

Medalia, O., Typke, D., Hegerl R., Angenitzki M., Sperling, J. & Sperling, R. Cryo-electron microscopy and cryo-electron tomography of the nuclear pre-mRNA processing machine. J. Struct. Biol138, 74-84 (2002).

Medalia, O., Englander, J., Guckenberger, R. & Sperling, J. Anchoring of DNA to a gold surface facilitates the imaging of protein-DNA complexes by atomic force microscopy in solution, Ultramicroscopy 90, 103-112 (2002).

2001
Henn, A., Medalia, O., Shu-Ping, S., Franceschi, F.  & Sagi, I. Demonstrating of unwinding activity of duplex RNA by DbpA, a DEAD box helicase, at single molecule level. Proc Natl. Acad. Sci. U.S.A  98,  5007-5012 (2001).

2000
Hölzl, H., Kapelari B,. Kellermann J., Seemuller E,. Sumegi M., Udvardy A., Medalia O., Sperling J, Muller S.A., Engel A. &  Baumeister, W. . The regulatory complex of Drosophila melanogaster 26S proteasomes: Subunit composition and localization of a deubiquitylating enzyme, J Cell Biol. 150, 119-130 (2000).

1999
Medalia, O., Heim, M., Guckenberger, R., Sperling, R. & Sperling, J.  Gold tagged RNA- A probe for RNP complexes, J. Struct. Biol127, 113-119 (1999).

1997
Medalia, O., Koster, A., Tocilj, A., Angenitzki, M., Sperling, J., Berkovitch-Yellin, Z. & Sperling, R. Automated electron tomography of large nuclear RNP (lnRNP) particles - the naturally assembled complexes of precursor messenger RNA and splicing factors.  J. Struct. Biol120, 228-236 (1997).