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Department of Biochemistry

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Publications of the Plückthun Group since 2011 (in alphabetical order)

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2018
Dreier, B. & Plückthun, A.Rapid selection of high-affinity antibody scFv fragments using ribosome display. Methods Mol Biol. 1827, 235-268 (2018). PMID: 30196501.

Ehrenmann, J., Schöppe, J., Klenk, C., Rappas, M., Kummer, L., Doré, AS. & Plückthun, A. High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist. Nat. Struct. Mol. Biol. 25, 1086-1092 (2018). PMID: 30455434.

ElGamacy M, Coles M, Ernst P, Zhu H, Hartmann MD, Plückthun A & Lupas AN. An interface-driven design strategy yields a novel , corrugated protein architecture. ACS Synth Biol. 7, 2226-2253 (2018). PMID: 30148951.

Hansen, S., Ernst, P., König, SLB., Reichen, C., Ewald, C., Nettels, D., Mittl, PRE., Schuler, B. & Plückthun, A. Curvature of deignes armadillo repeat proteins allows modular peptide binding. J Struct Biol. 201 108-117 (2018). PMID: 28864298.

Hartmann, J., Münch, RC., Freiling, RT., Schneider, IC., Dreier, B., Samukange, W., Koch, J., Seeger, MA., Plückthun, A. &  Buchholz, CJ. A library-based screening strategy for the identification of DARPins as ligands for receptor-targeted AAV and lentiviral vectors. Mol Ther Methods Clin Dev. 10, 128-143 (2018). PMID: 30101151.

Mikula, KM., Krumwiede, L., Plückthun, A. & Iwaï, H. Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation. J Biomol NMR 71, 225-235 (2018). PMID: 29536230.

Michel, E., Plückthun, A. &  Zerbe, O. Peptide-guided assembly of repeat protein fragments. Angew Chem Int Ed Engl. 57, 4576-4579 (2018). PMID: 29480529.

Radom, F., Plückthun, A. & Paci, E. Assessment of ab initio models of protein complexes by molecular dynamics. PLoS Comput Biol. 14, e 1006182 (2018). PMID: 29864135.

Schaefer, JV., Sedlák, E., Kast, F., Nemergut, M. & Plückthun, A. Modification of the kinetic stability of immunoglobulin G by solvent additives. MAbs. 10, 607-623 (2018). PMID: 29537925

Schmid, M., Ernst, P., Honegger, A., Suomalainen, M., Zimmermann, M., Braun, L., Stauffer, S., Thom, C., Dreier, B., Eibauer, M., Kipar, A., Vogel, V., Greber, UF., Medalia, O, & Plückthun, A. Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control. Nat Commun 9, 450 (2018). PMID: 29386504.

Stichling, N., Suomalainen, M., Flatt, JW., Schmid, M., Pacesa, M., Hemmi, S., Jungraithmayr, W., Maler, MD., Freudenberg, MA., Plückthun, A., May, T., Köster, M., Fejer, G. & Greber, UF. Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor. PLoS Pathog. 14 (3) e1006914 (2018). PMID: 29522575.

Vigano, MA., Bieli, D., Schaefer, JV., Jakob, RP., Matsuda, S., Maier, T., Plückthun, A. & Affolter, M. DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo manipulations. Biol Open. 7 (11) (2018). PMID: 30237292.

Wu,Y., Honegger, A., Batyuk, A., Mittl, P. & Plückthun, A. Structural basis for the selective inhibition of c-Jun N-terminal kinase 1 determined by rigid DARPin-DARPin fusions. J Mol Biol 430, 2128-2138 (2018). PMID: 29126898.

Yen, HY., Hoi, KK., Liko, I., Hedger, G., Horrell, MR., Song, W., Wu, D., Heine, P., Warne, T., Lee, Y., Carpenter, B., Plückthun, A., Tate, CG., Sansom, MSP. & Robinson, CV. PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling. Nature 559, 423-427 (2018). PMID: 29995853.

Yong, KJ., Vaid, TM., Shilling, PJ., Wu, FJ., Williams, LM., Deluigi, M., Plückthun, A., Bathgate, RA., Gooley, PR. & Scott, DJ. Determinants of ligand subtype-selectivity at alpha 1A-adrenoceptor revealed using saturation transfer difference (STD) NMR. ACS Chem Biol. 13, 1090-1102 (2018). PMID: 29537256.


2017
Afroz, T., Hock, EM., Ernst, P., Foglieni, C., Jambeau, M., Gilhespy, LAB., Laferriere, F., Maniecka, Z., Plückthun, A., Mittl, P., Paganetti, P., Allain, FHT. & Polymenidou, M. Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation. Nat Commun 8, 45 (2017). PMID: 28663553.

Anders, U., Schaefer, JV., Hibti, FE., Frydman, C., Suckau, D., Plückthun, A. & Zenobi, R. SPRi-MALDI MS: characterization and identification of a kinase from cell lysate by specific interaction with different designed ankyrin repeat proteins. Anal Bioanal Chem 409, 1827-1836 (2017). PMID: 27987025.

Ernst, P. & Plückthun, A. Advances in the design and engineering of peptidebinding repeat proteins. Biol. Chem. 398, 23-29 (2017). PMID: 27636831.

Hansen, S., Stüber, JC., Ernst, P., Koch, A., Bojar, D., Batyuk, A. & Plückthun, A. Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity. Sci Rep 7, 16292 (2017). PMID: 29176615.

Hansen, S., Kiefer, JD., Madhurantakam, C., Mittl, PRE. & Plückthun, A. Structures of designed armadillo repeat proteins binding to peptides fused to globular domains. Protein Sci. 26, 1942-1952 (2017). PMID: 28691351.

Huber, S., Casagrande, F., Hug, MN., Wang, L., Heine, P., Kummer, L., Plückthun, A. & Hennig, M. SPR-based fragment screening with neurotensin receptor 1 generates novel small molecule ligands. PLoS ONE 12, e0175842 (2017). PMID: 28510609.

Jost C., Stüber, JC., Honegger, A., Wu, Y., Batyuk, A. & Plückthun, A.  Rigidity of the ewxtracellular part of HER2: Evidence from engineering subdomain interfaces and shared-helix DARPin-DARPin fusions.  Protein Sci. 26, 1796-1806 (2017). PMID: 28639341.

Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, Plückthun A, Hagn F, Walz T, Hogle JM, Wagner G. Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nat Methods14, 49-52 (2017). PMID: 27869813.

Nemergut, M., Žoldák, G., Schaefer, JV., Kast, F., Miškovský, P., Plückthun, A. & Sedlák, E. Analysis of IgG kinetic stability by differential scanning calorimetry, probe fluorescence and light scattering. Protein Sci. 26, 2229-2239 (2017). PMID: 28833802.

Obexer R, Godina A, Garrabou X, Mittl PR, Baker D, Griffiths AD, Hilvert D. Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase. Nat Chem.9, 50-56 (2017). PMID: 27995916.

Parren, PWHI., Carter, PJ. & Plückthun, A. Changes to International Nonproprietary Names for antibody therapeutics 2017 and beyond: of mice, men and more. MAbs 9, 898-906 (2017). PMID: 28621572.

Ranganathan, A., Heine, P., Rudling, A., Plückthun, A., Kummer, L. & Carlsson, J. Ligand Discovery for a Peptide-Binding GPCR by Structure-Based Screening of Fragment- and Lead-Like Chemical Libraries. ACS Chem. Biol. 12, 735-745 (2017). PMID: 28032980.

Syafrizayanti, Lueong SS, Di C, Schaefer JV, Plückthun A, Hoheisel JD. Personalised proteome analysis by means of protein microarrays made from individual patient samples. Sci Rep. 7, 39756 (2017). PMID: 28045055.

Verdurmen, WPR., Mazlami, M. & Plückthun, A. A quantitative comparison of cytosolic delivery via different protein uptake systems. Sci Rep 7, 13194 (2017). PMID: 29038564.

Verdurmen, WP., Mazlami, M. & Plückthun, A. A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins. Methods Mol. Biol. 1575, 223-236 (2017). PMID: 28255884.

Wu, Y., Batyuk, A., Honegger, A., Brandl, F., Mittl, PRE. & Plückthun, A. Rigidly connected multispecific artificial binders with adjustable geometries. Sci Rep 7, 11217 (2017). PMID: 28894181.

2016
Ahmad, S., Pecqueur, L., Dreier, B., Hamdane, D., Aumont-Nicaise, M., Plückthun, A., Knossow, M. & Gigant, B. Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin. Sci Rep 6, 28922 (2016). PMID: 27380724.

Batyuk, A., Wu, Y., Honegger, A., Heberling, MM. & Plückthun, A. DARPin-based crystallization chaperones exploit molecular geometry as a screening dimension in protein crystallography. J. Mol. Biol. 428, 1574-88 (2016). PMID: 26975886.

Bender RR, Muth A, Schneider IC, Friedel T, Hartmann J, Plückthun A, Maisner A, Buchholz CJ. Receptor-targeted Nipah virus glycoproteins improve cell-type selective gene delivery and reveal a preference for membrane-proximal cell attachment. PLoS Pathog. 12, e1005641 (2016) PMID: 27281338.

Ernst, P. & Plückthun, A. Advances in the design and engineering of peptide binding repeat proteins. Biol. Chem. 398, 23-29 (2016). PMID: 27636831.

Goricanec, D., Stehle, R., Egloff, P., Grigoriu, S., Plückthun, A., Wagner, G. & Hagn, F. Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding. Proc. Natl. Acad. Sci. U.S.A. 113, E3629-38 (2016). PMID: 27298341.

Hanauer, JR., Gottschlich, L., Riehl, D., Rusch, T., Koch, V., Friedrich, K., Hutzler, S., Prüfer, S., Friedel, T., Hanschmann, KM., Münch, RC., Jost, C., Plückthun, A., Cichutek, K., Buchholz, CJ. & Mühlebach, MD. Enhanced lysis by bispecific oncolytic measles viruses simultaneously using HER2/neu or EpCAM as target receptors. Mol Ther Oncolytics 3, 16003 (2016). PMID: 27119117.

Hansen, S., Tremmel, D., Madhurantakam, C., Reichen, C., Mittl, PR. & Plückthun, A. Structure and energetic contributions of a designed modular peptide-binding protein with picomolar affinity. J. Am. Chem. Soc. 138, 3526-32 (2016). PMID: 26878586.

Jones, TD., Carter, PJ., Plückthun, A., Vásquez, M., Holgate, RG., Hötzel, I., Popplewell, AG., Parren, PW., Enzelberger, M., Rademaker, HJ., Clark, MR., Lowe, DC., Dahiyat, BI., Smith, V., Lambert, JM., Wu, H., Reilly, M., Haurum, JS., Dübel, S., Huston, JS., Schirrmann, T., Janssen, RA., Steegmaier, M., Gross, JA., Bradbury, AR., Burton, DR., Dimitrov, DS., Chester, KA., Glennie, MJ., Davies, J., Walker, A., Martin, S., McCafferty, J. & Baker, MP. The INNs and outs of antibody nonproprietary names. MAbs 8, 1-9 (2016). PMID: 26716992.

Klenk, C., Ehrenmann, J., Schütz, M. & Plückthun, A. A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution. Sci Rep 6, 21294 (2016). PMID: 26887595.

Reichen, C., Hansen, S., Forzani, C., Honegger, A., Fleishman, SJ., Zhou, T., Parmeggiani, F., Ernst, P., Madhurantakam, C., Ewald, C., Mittl, PR., Zerbe, O., Baker, D., Caflisch, A. & Plückthun, A. Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition. J. Mol. Biol. 428, 4467-4489 (2016). PMID: 27664438.

Reichen, C., Madhurantakam, C., Hansen, S., Grütter, MG., Plückthun, A. & Mittl, PR. Structures of designed armadillo-repeat proteins show propagation of inter-repeat interface effects. Acta Crystallogr D Struct Biol 72, 168-75 (2016). PMID: 26894544.

Schütz, M., Schöppe, J., Sedlák, E., Hillenbrand, M., Nagy-Davidescu, G., Ehrenmann, J., Klenk, C., Egloff, P., Kummer, L. & Plückthun, A. Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts. Sci Rep 6, 21508 (2016). PMID: 26911446.

Schütz, M., Batyuk, A., Klenk, C., Kummer, L., de Picciotto, S., Gülbakan, B., Wu, Y., Newby, GA., Zosel, F., Schöppe, J., Sedlák, E., Mittl, PR., Zenobi, R., Wittrup, KD. & Plückthun, A. Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools. J. Mol. Biol.  428, 1272-89 (2016). PMID: 26812208.

Tamaskovic, R., Schwill, M., Nagy-Davidescu, G., Jost, C., Schaefer, DC., Verdurmen, WP., Schaefer, JV., Honegger, A. & Plückthun, A. Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS-p110 crosstalk. Nat Commun 7, 11672 (2016). PMID: 27255951
  
2015
Bradbury, A. & Plückthun, A. Reproducibility: Standardize antibodies used in research. Nature 518, 27-9 (2015). PMID: 25652980.

Bradbury, AM. & Plückthun, A. Antibodies: validate recombinants once. Nature 520, 295 (2015). PMID: 25877194.

Bradbury, AR. & Plückthun, A. Getting to reproducible antibodies: the rationale for sequenced recombinant characterized reagents. Protein Eng. Des. Sel. 28, 303-5 (2015). PMID: 26446960.

Egloff, P., Deluigi, M., Heine, P., Balada, S. & Plückthun, A. A cleavable ligand column for the rapid isolation of large quantities of homogeneous and functional neurotensin receptor 1 variants from E. coli. Protein Expr. Purif. 108, 106-14 (2015). PMID: 25461958.

Ewald, C., Christen, MT., Watson, RP., Mihajlovic, M., Zhou, T., Honegger, A., Plückthun, A., Caflisch, A. & Zerbe, O. A combined NMR and computational approach to investigate peptide binding to a designed Armadillo repeat protein. J. Mol. Biol. 427, 1916-33 (2015). PMID: 25816772.

Fleishman, SJ. & Plückthun, A. Editorial overview: Protein design and evolution-new protein architectures, evolutionary fine-tuning and analysis. Curr. Opin. Struct. Biol. 33, v-vi (2015). PMID: 26433320.

Friedel, T., Hanisch, LJ., Muth, A., Honegger, A., Abken, H., Plückthun, A., Buchholz, CJ. & Schneider, IC. Receptor-targeted lentiviral vectors are exceptionally sensitive toward the biophysical properties of the displayed single-chain Fv. Protein Eng. Des. Sel. 28, 93-106 (2015). PMID: 25715658.

Goldstein, R., Sosabowski, J., Livanos, M., Leyton, J., Vigor, K., Bhavsar, G., Nagy-Davidescu, G., Rashid, M., Miranda, E., Yeung, J., Tolner, B., Plückthun, A., Mather, S., Meyer, T. & Chester, K. Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging. Eur. J. Nucl. Med. Mol. Imaging 42, 288-301 (2015). PMID: 25391547.

Hillenbrand, M., Schori, C., Schöppe, J. & Plückthun, A. Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution. Proc. Natl. Acad. Sci. U.S.A. 112, E1181-90 (2015). PMID: 25733868.

König, I., Zarrine-Afsar, A., Aznauryan, M., Soranno, A., Wunderlich, B., Dingfelder, F., Stüber, JC., Plückthun, A., Nettels, D. & Schuler, B. Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells. Nat. Methods 12, 773-9 (2015). PMID: 26147918.

Merten, H., Brandl, F., Plückthun, A. & Zangemeister-Wittke, U. Antibody-Drug Conjugates for Tumor Targeting-Novel Conjugation Chemistries and the Promise of non-IgG Binding Proteins. Bioconjug. Chem. 26, 2176-85 (2015). PMID: 26086208.

Münch, RC., Muth, A., Muik, A., Friedel, T., Schmatz, J., Dreier, B., Trkola, A., Plückthun, A., Büning, H. & Buchholz, CJ. Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors. Nat Commun 6, 6246 (2015). PMID: 25665714.

Oude Blenke, E., Klaasse, G., Merten, H., Plückthun, A., Mastrobattista, E. & Martin, NI. Liposome functionalization with copper-free "click chemistry". J Control Release 202, 14-20 (2015). PMID: 25626085.

Plückthun, A. Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy. Annu. Rev. Pharmacol. Toxicol. 55, 489-511 (2015). PMID: 25562645.

Salvati Manni, L., Zabara, A., Osornio, YM., Schöppe, J., Batyuk, A., Plückthun, A., Siegel, JS., Mezzenga, R. & Landau, EM. Phase behavior of a designed cyclopropyl analogue of monoolein: implications for low-temperature membrane protein crystallization. Angew. Chem. Int. Ed. Engl. 54, 1027-31 (2015). PMID: 25418121.

Sedlák, E., Schaefer, JV., Marek, J., Gimeson, P. & Plückthun, A. Advanced analyses of kinetic stabilities of iggs modified by mutations and glycosylation. Protein Sci. 24, 1100-13 (2015). PMID: 25966898.

Verdurmen, WP., Luginbühl, M., Honegger, A. & Plückthun, A. Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems. J Control Release 200, 13-22 (2015). PMID: 25526701.

2014
Brauchle, M., Hansen, S., Caussinus, E., Lenard, A., Ochoa-Espinosa, A., Scholz, O., Sprecher, SG., Plückthun, A. & Affolter, M. Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry. Biol Open 3, 1252-61 (2014). PMID: 25416061.

Egloff, P., Deluigi, M., Heine, P., Balada, S. & Plückthun, A. A cleavable ligand column for the rapid isolation of large quantities of homogeneous and functional neurotensin receptor 1 variants from E. coli. Protein Expr. Purif. [Epub ahead of print] (2014). PMID: 25461958.

Egloff, P., Hillenbrand, M., Klenk, C., Batyuk, A., Heine, P., Balada, S., Schlinkmann, KM., Scott, DJ., Schütz, M. & Plückthun, A. Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 111, E655-62 (2014). PMID: 24453215.

Goldstein, R., Sosabowski, J., Livanos, M., Leyton, J., Vigor, K., Bhavsar, G., Nagy-Davidescu, G., Rashid, M., Miranda, E., Yeung, J., Tolner, B., Plückthun, A., Mather, S., Meyer, T. & Chester, K. Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging. Eur. J. Nucl. Med. Mol. Imaging [Epub ahead of print] (2014). PMID: 25391547.

Hanenberg, M., McAfoose, J., Kulic, L., Welt, T., Wirth, F., Parizek, P., Strobel, L., Cattepoel, S., Späni, C., Derungs, R., Maier, M., Plückthun, A. & Nitsch, RM. Aβ-specific DARPins as a novel class of potential therapeutics for Alzheimer's disease. J. Biol. Chem. 289, 27080-9 (2014). PMID: 25118284.

Jost, C. & Plückthun, A. Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs. Curr. Opin. Struct. Biol. 27, 102-12 (2014). PMID: 25033247.

Kiuchi, T., Ortiz-Zapater, E., Monypenny, J., Matthews, DR., Nguyen, LK., Barbeau, J., Coban, O., Lawler, K., Burford, B., Rolfe, DJ., de Rinaldis, E., Dafou, D., Simpson, MA., Woodman, N., Pinder, S., Gillett, CE., Devauges, V., Poland, SP., Fruhwirth, G., Marra, P., Boersma, YL., Plückthun, A., Gullick, WJ., Yarden, Y., Santis, G., Winn, M., Kholodenko, BN., Martin-Fernandez, ML., Parker, P., Tutt, A., Ameer-Beg, SM. & Ng, T. The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility. Sci Signal 7, ra78 (2014). PMID: 25140053.

Reichen, C., Hansen, S. & Plückthun, A. Modular peptide binding: From a comparison of natural binders to designed armadillo repeat proteins. J. Struct. Biol. 185, 147-62 (2014). PMID: 23916513.

Reichen, C., Madhurantakam, C., Plückthun, A. & Mittl, PR. Crystal structures of designed armadillo repeat proteins: Implications of construct design and crystallization conditions on overall structure. Protein Sci.  23, 1572-83 (2014). PMID: 25132085.

Schilling, J., Schöppe, J., Sauer, E. & Plückthun, A. Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W. J. Mol. Biol. 426, 2346-62 (2014). PMID: 24747052.

Schilling, J., Schöppe, J. & Plückthun, A. From DARPins to LoopDARPins: novel LoopDARPin design allows the selection of low picomolar binders in a single round of ribosome display. J. Mol. Biol. 426, 691-721 (2014). PMID: 24513107.

Scholz, O., Hansen, S. & Plückthun, A. G-quadruplexes are specifically recognized and distinguished by selected designed ankyrin repeat proteins. Nucleic Acids Res. 42, 9182-94 (2014). PMID: 25053846.

Scott, DJ., Kummer, L., Egloff, P., Bathgate, RA. & Plückthun, A. Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies. Biochim. Biophys. Acta 1838, 2817-24 (2014). PMID: 25064156.

Simon, M., Stefan, N., Borsig, L., Plückthun, A. & Zangemeister-Wittke, U. Increasing the Antitumor Effect of an EpCAM-Targeting Fusion Toxin by Facile Click PEGylation. Mol. Cancer Ther. 13, 375-85 (2014). PMID: 24184633.

Stefan, N., Zimmermann, M., Simon, M., Zangemeister-Wittke, U. & Plückthun, A. A novel prodrug-like fusion toxin with protease-sensitive bioorthogonal PEGylation for tumor targeting. Bioconjug. Chem.  25, 2144-56 (2014). PMID: 25350699.

Watson, RP., Christen, MT., Ewald, C., Bumbak, F., Reichen, C., Mihajlovic, M., Schmidt, E., Güntert, P., Caflisch, A., Plückthun, A. & Zerbe, O. Spontaneous Self-Assembly of Engineered Armadillo Repeat Protein Fragments into a Folded Structure. Structure 22, 985-95 (2014). PMID: 24931467.

Yan, J., Gu, GJ., Jost, C., Hammond, M., Plückthun, A., Landegren, U. & Kamali-Moghaddam, M. A Universal Approach to Prepare Reagents for DNA-Assisted Protein Analysis. PLoS ONE 9, e108061 (2014). PMID: 25233463.

2013
Dreier, B., Honegger, A., Hess, C., Nagy-Davidescu, G., Mittl, PR., Grütter, MG., Belousova, N., Mekheeva, G., Krasnykh, V. & Plückthun, A. Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. PNAS USA 110, E869-877 (2013).

Epa, VC., Dolezal, O., Doughthy, L., Xiao, X., Jost, C., Plückthun. A. & Adams, TE. Structural model for the interaction of a designed ankyrin repeat protein with the human epidermal growth factor receptor 2. PLoS One. 8(3): e59163 (2013).

Friedrich, K., Hanauer, JR., Prüfer, S., Münch, RC., Völker, I., Filippis, C., Jost, C., Hanschmann, KM., Cattaneo, R., Peng, KW., Plückthun, A., Buchholz, CJ., Cichutek, K. & Mühlebach, MD. DARPin-targeting of measles virus: unique bispecificity, effective oncolysis, and enhanced safety. Mol Ther. 21, 849-859 (2013).

Gigant, B., Wang, W., Dreier, B., Jiang, Q., Pecqueur, L., Plückthun, A., Wang, C. & Knossow, M. Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat Struct Mol Biol. 20, 1001-1007 (2013).

Jost, C., Schilling, J., Tamaskovic, R., Schwill, M., Honegger, A. & Plückthun, A. Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. Structure 21, 1979-91 (2013).

Kummer, L., Hsu, CW., Dagliyan, O., Macnevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, NV., Hahn, KM. & Plückthun, A. Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. Chem Biol. 20, 847-856 (2013).

Mann, A., Friedrich, N., Krarup, A., Weber, J., Stiegeler, E., Dreier, B., Pugach, P., Robbiani, M., Riedel, T., Moehle, K., Robinson, JA., Rusert, P., Plückthun, A. & Trkola, A. Conformation-dependent recognition of HIV gp 120 by designed ankyrin repeat proteins provides access to novel HIV entry inhibitors. J Virol. 87, 5868-5881 (2013).

Schlinkmann, KM. & Plückthun. A. Directed evolution of g-coupled receptors for high functional expression and detergent stability. Methods Enzymol. 520, 67-97 (2013).

Scott, D. & Plückthun, A. Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. J Mol Biol 425, 662-677 (2013).

Scott, D., Kummer, L., Tremmel, D. & Plückthun, A. Stabilizing membrane proteins through protein engineering. Curr Opin Chem Biol. 17, 427-435 (2013).

Simon, M., Stefan. N., Plückthun, A. & Zangemesiter-Wittke, U. Epithelial cell adhesion molecule-targeted drug delivery for cancer therapy. Expert Opin Drug Deliv. 10, 451-468 (2013).

Simon, M., Frey, R., Zangemeister-Wittke, U. & Plückthun, A. Orthogonal assembly of a designed ankyrin repeat protein-cytotoxin conjugate with a clickable serum albumin module for half-life extension. Bioconjug. Chem. 24, 1955-66 (2013)

2012
Alfarano, P., Varadamsetty, G., Ewald, C., Parmeggiani, F., Pellarin, R., Zerbe, O., Plückthun, A. & Caflisch, A. Optimization of designed armadillo repeat proteins by molecular dynamics simulations and NMR spectroscopy. Protein Sci. 21, 1298-1314 ( 2012).

Dreier, B. & Plückthun, A. Rapid selection of high-affinity binders using ribosome display. Methods Mol. Biol. 805, 261-286 (2012).

Frei, AP., Jeon, OY., Kilcher, S., Moest, H., Henning, LM., Jost, C., Plückthun, A., Mercer, J., Aebersold, R., Carreira, EM. & Wollscheid, B. Direct identification of ligand-receptor interactions on living cells and tissues. Nat Biotechnol. 30, 997-1001 (2012).

Gu, GJ., Friedmann, M., Jost, C., Johnsson, K., Kamali-Moghaddam, M., Plückthun, A., Landegren, U. & Söderberg, O. Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N Biotechnol. 2012 Jun 2. [Epub ahead of print]

Kummer, L.,Parizek, P., Rube, P., Millgramm, B., Prinz, A., Mittl, PR., Kaufholz, M., Zimmermann, B., Herberg, FW. & Plückthun, A. Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. PNAS USA. 109, E2248-2257 (2012).

Madhurantakam, C., Varadamsetty, G., Grütter, MG., Plückthun, A. & Mittl, PR. Structure-based optimization of designed Armadillo-repeat proteins. Protein Sci. 21, 1015-1028 (2012).

Mignot,I., Pecqueur, L., Dorléans, A., Karuppasamy, M., Ravelli, RB., Dreier, B., Plückthun, A., Knossow, M. & Gigant, B. Design and characterization of modular scaffolds for tubulin assembly. J Biol Chem. 287, 31085-31094 (2012).

Parizek, P., Kummer, L., Rube, P., Prinz, A., Herberg, FW. & Plückthun, A. Ankyrin repeat proteins (DARPins) as novel isoform-specific intracellular inhibitors of c-jun-N-terminal kinases. ACS Chem Biol.  7, 1356-1366 (2012).

Pecqueur, L., Duellberg, C., Dreier, B., Jiang, Q., Wang, C., Plückthun, A., Surrey, T., Gigant, B. & Knossow, M. A designed ankyrin repeat protein selected to bind to tubulin caps at the microtubule plus end. Proc Natl Acad Sci U S A. 109, 12011-12016 (2012).

Plückthun, A. Ribosome display: a perspective. Methods Mol. Biol. 805, 3-28 (2012).

Schaefer, JV. & Plückthun, A. Transfer of engineered biophysical properties between different antibody formats and expression systems. Protein Eng Des Sel. 25, 485-506 (2012).

Schaefer, JV. & Plückthun, A. Engineering aggregation resistance in IgG by two independent mechanisms: lessons from comparison of pichia pastoris and mammalian cell expression. J Mol Biol. 417, 309-335 (2012).

Schlinkmann, KM., Hillenbrand, M., Rittner, A., Künz, M., Strohner, R. & Plückthun, A. Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. J Mol Biol. 422, 414-428 (2012).

Schlinkmann, KM., Honegger, A., Türeci, E., Robison, KE., Lipovsek, D. & Plückthun, A. Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor. PNAS USA 109, 9810-9815 (2012). 

Simon, M., Zangemeister-Wittke, U. & Plückthun, A. Facile double-functionalization of designed Ankyrin repeat proteins using click and thiol chemistries. Bioconjug Chem. 23, 279-286 (2012).

Tamaskovic, R., Simon, M., Stefan, N., Schwill, M. & Plückthun, A. Designed Ankyrin Repeat Proteins (DARPins): From Research to Therapy. Methods Enzymol. 503, 101-134 (2012). 

Varadamsetty, G., Tremmel, D., Hansen, S., Parmeggiani, F. & Plückthun, A. Designed armadillo repeat proteins: library generation, characterization and selection of peptide binders with high specificity. J Mol Biol. 424, 68-87 (2012).

Zhou, Q., Schneider, IC., Edes, I., Honegger, A., Bach, P., Schönfeld, K., Schambach, A., Wels, WS., Kneissl, S., Uckert, W. & Buchholz, CJ. T cell receptor gene transfer exclusively to human CD8+ cells enhances tumor cell killing. Blood 120, 4334-4442 (2012).

2011
Boersma, YL. & Plückthun, A. DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr Opin Biotechnol. 22, 849-857 (2011).

Boersma, Y., Chao, G., Steiner, D., Wittrup, KD. & Plückthun, A. Bispecific Designed Ankyrin repeat Proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol. Chem. 286, 41273-41285 (2011). 

Dreier, B. & Plückthun, A. Ribosome display: a technology for selecting and evolving proteins from large libraries. Methods Mol. Biol. 687, 283-306 (2011).

Dreier, B., Mikheeva, G., Belousova, N., Parizek, P., Boczek, E., Jelesarov, I., Forrer, P., Plückthun, A. & Krasnykh, V. Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting. J. Mol. Biol.  405, 410-426 (2011).

Dodevski, I. & Plückthun, A. Evolution of three human GPCRs for higher expression and stability. J Mol. Biol. 408, 599-615 (2011).

Huwiler, A., Bourquin, F., Kotelevets, N., Pastukhov, O., Capitani, G., Grütter, MG. & Zangemeister-Wittke, U. A prokaryotic S1P lyase degrades extracellular S1P in vitro and in vivo: implication for treating hyperproliferative disorders. PLoS One. 6(8): e22436 (2011). 

Martin-Killias, P., Stefan, N., Rothschild, S., Plückthun, A. & Zangemeister-Wittke, U. A novel fusion toxin derived from an EpCAM-specific designed ankyrin repeat protein has potent anti-tumor activity. Clin Cancer Res. 17, 100-110 (2011). 

Münch, RC., Mühlebach, MC., Schaser, T., Kneissl, S., Jost, C., Plückthun, A., Cichutek, K. & Buchholz, CJ. DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther. 19, 686-693 (2011). 

Stefan, N., Martin-Killias, P., Wyss-Stoeckle, S., Honegger, A., Zangemeister-Wittke, U. & Plückthun, A. DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. J Mol Biol. 413, 826-843 (2011). 

Stevens, GB., Krüger, M., Latychevskaia, T., Lindner, P., Plückthun, A. & Fink, HW. Individual filamentous phage imaged by electron holography. Eur Biophys J. 40, 1197-1201 (2011).

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