Sample Volume

Vapor diffusion crystallization experiment

The Phoenix and Gryphon-LCP liquid handling system can routinely dispense a drop volume between 100nl and 250nl for both the protein sample and reservoir solution into the well of a MRC 3-lens crystallization plate. Up to three different protein samples can be dispensed on a single MRC 3-lens crystallization plate. The protein sample volume needed for the crystallization experiments depends on the drop volume and the number of plates:

 

Drop volume Sample volume per plate Sample volume for N plates
100 nl 9.6 ul N*9.6 +20 ul
150 nl 14.4 ul N*14.4 +20 ul
... ... ...
250 nl 24.0 ul N*24.0 +20 ul

Example of sample volume calculation:

The sample volume required for 4 plates (96 Well), 3 drops of (100nl, 150nl, 200nl)  is:
(4 x (0.1+0.15+0.2)uL x 96) +20 =192.8 uL

 

Note:

Leftover protein samples will be stored at 4°C for up to one month if indicated in the reservation calendar. Samples older than 1 month will be discarded without notice.

Microseeding crystallization experiment

Seedstock drop volume Seedstock volume per plate Seedstock volume for N plate
20 nl >2.5 ul N*2.5 +20 ul
50 nl 5.0 ul N*5.0 +20 ul