Publications of the Kägi Group

Orihuela, R., Domènech, J., Bofill, R., You, C., Kägi, JH., Capdevila, M. & Atrian, S. The metal-binding features of the recombinant mussel Mytilus edulis MT-10-IV metallothionein. J Biol Inorg Chem. 13, 801-812 (2008).  

Dallinger, R., Wang, Y., Berger, B., Mackay, E. A. & Kagi, J. H. Spectroscopic characterization of metallothionein from the terrestrial snail, Helix pomatia. Eur. J. Biochem. 268, 4126–33 (2001).    
    
Gehrig, P. M., You, C., Dallinger, R., Gruber, C., Brouwer, M., Kagi, J. H. & Hunziker, P. E. Electrospray ionization mass spectrometry of zinc, cadmium, and copper metallothioneins: evidence for metal-binding cooperativity. Protein Sci. 9, 395–402 (2000).    
    
Riek, R., Precheur, B., Wang, Y., Mackay, E. A., Wider, G., Guntert, P., Liu, A., Kagi, J. H. & Wuthrich, K. NMR structure of the sea urchin (Strongylocentrotus purpuratus) metallothionein MTA. J. Mol. Biol. 291, 417–28 (1999).    
    
You, C., Mackay, E. A., Gehrig, P. M., Hunziker, P. E. & Kagi, J. H. Purification and characterization of recombinant Caenorhabditis elegans metallothionein. Arch. Biochem. Biophys. 372, 44–52 (1999).    
   
Roesijadi, G., Bogumil, R., Vasak, M. & Kagi, J. H. Modulation of DNA binding of a tramtrack zinc finger peptide by the metallothionein-thionein conjugate pair. J. Biol. Chem. 273, 17425–32 (1998).    
    
Dallinger, R., Berger, B., Hunziker, P. & Kagi, J. H. Metallothionein in snail Cd and Cu metabolism. Nature 388, 237–8 (1997).    
    
Studer, R., Vogt, C. P., Cavigelli, M., Hunziker, P. E. & Kagi, J. H. Metallothionein accretion in human hepatic cells is linked to cellular proliferation. Biochem. J. 328 ( Pt 1), 63–7 (1997).    
    
Wang, Y., Hess, D., Hunziker, P. E. & Kagi, J. H. Separation and characterization of the metal-thiolate-cluster domains of recombinant sea urchin metallothionein. Eur. J. Biochem. 241, 835–9 (1996).    
   
Wan, M., Heuchel, R., Radtke, F., Hunziker, P. E. & Kagi, J. H. Regulation of metallothionein gene expression in Cd- or Zn-adapted RK-13 cells. Experientia 51, 606–11 (1995).    
    
Wang, Y., Mackay, E. A., Zerbe, O., Hess, D., Hunziker, P. E., Vasak, M. & Kagi, J. H. Characterization and sequential localization of the metal clusters in sea urchin metallothionein. Biochemistry 34, 7460–7 (1995).    
   
Wang, Y., Mackay, E. A., Kurasaki, M. & Kagi, J. H. Purification and characterisation of recombinant sea urchin metallothionein expressed in Escherichia coli. Eur. J. Biochem. 225, 449–57 (1994).    
    
Cavigelli, M., Kagi, J. H. & Hunziker, P. E. Cell- and inducer-specific accretion of human isometallothioneins. Biochem. J. 292 ( Pt 2), 551–4 (1993).    
    
Dallinger, R., Berger, B., Hunziker, P. E., Birchler, N., Hauer, C. R. & Kagi, J. H. Purification and primary structure of snail metallothionein. Similarity of the N-terminal sequence with histones H4 and H2A. Eur. J. Biochem. 216, 739–46 (1993).    
    
Mackay, E. A., Overnell, J., Dunbar, B., Davidson, I., Hunziker, P. E., Kagi, J. H. & Fothergill, J. E. Complete amino acid sequences of five dimeric and four monomeric forms of metallothionein from the edible mussel Mytilus edulis. Eur. J. Biochem. 218, 183–94 (1993).    
    
Wan, M., Hunziker, P. E. & Kagi, J. H. Induction of metallothionein synthesis by cadmium and zinc in cultured rabbit kidney cells (RK-13). Biochem. J. 292 ( Pt 2), 609–15 (1993).    
    
Wan, M., Kagi, J. H. & Hunziker, P. E. Resolution and quantification of four metallothionein isoforms from rabbit kidney cells. Protein Expr. Purif. 4, 38–44 (1993).    
    
Braun, W., Vasak, M., Robbins, A. H., Stout, C. D., Wagner, G., Kagi, J. H. & Wuthrich, K. Comparison of the NMR solution structure and the x-ray crystal structure of rat metallothionein-2. Proc. Natl Acad. Sci. U. S. A. 89, 10124–8 (1992).    
    
Messerle, B. A., Schaffer, A., Vasak, M., Kagi, J. H. & Wuthrich, K. Comparison of the solution conformations of human [Zn7]-metallothionein-2 and [Cd7]-metallothionein-2 using nuclear magnetic resonance spectroscopy. J. Mol. Biol. 225, 433–43 (1992).    
    
Plocke, D. J. & Kagi, J. H. Spectral characteristics of cadmium-containing phytochelatin complexes isolated from Schizosaccharomyces pombe. Eur. J. Biochem. 207, 201–5 (1992).    
    
Kagi, J. H. Overview of metallothionein. Methods Enzymol. 205, 613–26 (1991).    
    
Zeng, J., Heuchel, R., Schaffner, W. & Kagi, J. H. Thionein (apometallothionein) can modulate DNA binding and transcription activation by zinc finger containing factor Sp1. FEBS Lett279, 310–2 (1991).    
    
Zeng, J., Vallee, B. L. & Kagi, J. H. Zinc transfer from transcription factor IIIA fingers to thionein clusters. Proc. Natl. Acad. Sci. U. S. A. 88, 9984–8 (1991).

Messerle, B. A., Bos, M., Schaffer, A., Vasak, M., Kagi, J. H. & Wuthrich, K. Amide proton exchange in human metallothionein-2 measured by nuclear magnetic resonance spectroscopy. J. Mol. Biol. 214, 781–6 (1990).    
    
Messerle, B. A., Schaffer, A., Vasak, M., Kagi, J. H. & Wuthrich, K. Three-dimensional structure of human [113Cd7]metallothionein-2 in solution determined by nuclear magnetic resonance spectroscopy. J. Mol. Biol. 214, 765–79 (1990).    
    
Sudfeldt, C., Schaffer, A., Kagi, J. H., Bogumil, R., Schulz, H. P., Wulff, S. & Witzel, H. Spectroscopic studies on the metal-ion-binding sites of Co2(+)-substituted D-xylose isomerase from Streptomyces rubiginosus. Eur. J. Biochem. 193, 863–71 (1990).        

Kagi, J. H. & Hunziker, P. Mammalian metallothionein. Biol. Trace Elem. Res. 21, 111–8 (1989).    
    
Arseniev, A., Schultze, P., Worgotter, E., Braun, W., Wagner, G., Vasak, M., Kagi, J. H. & Wuthrich, K. Three-dimensional structure of rabbit liver [Cd7]metallothionein-2a in aqueous solution determined by nuclear magnetic resonance. J. Mol. Biol. 201, 637–57 (1988).    
    
Kagi, J. H. & Schaffer, A. Biochemistry of metallothionein. Biochemistry 27, 8509–15 (1988).    
    
Schultze, P., Worgotter, E., Braun, W., Wagner, G., Vasak, M., Kagi, J. H. & Wuthrich, K. Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy. J. Mol. Biol. 203, 251–68 (1988).    
    
Bernhard, W. R. & Kagi, J. H. Purification and characterization of atypical cadmium-binding polypeptides from Zea mays. Experientia Suppl. 52, 309–15 (1987).    
    
Bernhard, W. R., Vasak, M. & Kagi, J. H. Cadmium binding and metal cluster formation in metallothionein: a differential modification study. Experientia Suppl. 52, 243–6 (1987).    
    
De Weck, Z., Pande, J. & Kagi, J. H. Interdependence of coenzyme-induced conformational work and binding potential in yeast alcohol and porcine heart lactate dehydrogenases: a hydrogen-deuterium exchange study. Biochemistry 26, 4769–76 (1987).    
    
Hunziker, P. E. & Kagi, J. H. Human hepatic metallothioneins: resolution of six isoforms. Experientia Suppl. 52, 257–64 (1987).    
    
Kagi, J. H. & Kojima, Y. Chemistry and biochemistry of metallothionein. Experientia Suppl. 52, 25–61 (1987).    
    
Vasak, M., Worgotter, E., Wagner, G., Kagi, J. H. & Wuthrich, K. Metal co-ordination in rat liver metallothionein-2 prepared with or without reconstitution of the metal clusters, and comparison with rabbit liver metallothionein-2. J. Mol. Biol. 196, 711–9 (1987).    
    
Wagner, G., Frey, M. H., Neuhaus, D., Worgotter, E., Braun, W., Vasak, M., Kagi, J. H. & Wuthrich, K. Spatial structure of rabbit liver metallothionein-2 in solution by NMR. Experientia Suppl. 52, 149–57 (1987).    
    
Willner, H., Vasak, M. & Kagi, J. H. Cadmium-thiolate clusters in metallothionein: spectrophotometric and spectropolarimetric features. Biochemistry 26, 6287–92 (1987).    
    
Worgotter, E., Wagner, G., Vasak, M., Kagi, J. H. & Wuthrich, K. Sequence-specific 1H-NMR assignments in rat-liver metallothionein-2. Eur. J. Biochem. 167, 457–66 (1987).    
    
Bernhard, W. R., Vasak, M. & Kagi, J. H. Cadmium binding and metal cluster formation in metallothionein: a differential modification study. Biochemistry 25, 1975–80 (1986).    
    
Braun, W., Wagner, G., Worgotter, E., Vasak, M., Kagi, J. H. & Wuthrich, K. Polypeptide fold in the two metal clusters of metallothionein-2 by nuclear magnetic resonance in solution. J. Mol. Biol. 187, 125–9 (1986).    
    
Glennas, A., Hunziker, P. E., Garvey, J. S., Kagi, J. H. & Rugstad, H. E. Metallothionein in cultured human epithelial cells and synovial rheumatoid fibroblasts after in vitro treatment with auranofin. Biochem. Pharmacol. 35, 2033–40 (1986).    
    
Pande, J., Pande, C., Gilg, D., Vasak, M., Callender, R. & Kagi, J. H. Raman, infrared, and circular dichroism spectroscopic studies on metallothionein: a predominantly "turn"-containing protein. Biochemistry 25, 5526–32 (1986).    
    
Wagner, G., Neuhaus, D., Worgotter, E., Vasak, M., Kagi, J. H. & Wuthrich, K. Sequence-specific 1H-NMR assignments in rabbit-liver metallothionein-2. Eur. J. Biochem. 157, 275–89 (1986).    
    
Wagner, G., Neuhaus, D., Worgotter, E., Vasak, M., Kagi, J. H. & Wuthrich, K. Nuclear magnetic resonance identification of "half-turn" and 3(10)-helix secondary structure in rabbit liver metallothionein-2. J. Mol. Biol. 187, 131–5 (1986).    
    
Frazier, J. M., George, S. S., Overnell, J., Coombs, T. L. & Kagi, J. Characterization of two molecular weight classes of cadmium binding proteins from the mussel, Mytilus edulis (L.). Comp. Biochem. Physiol. C 80, 257–62 (1985).    
    
Hunziker, P. E. & Kagi, J. H. Isolation and characterization of six human hepatic isometallothioneins. Biochem. J. 231, 375–82 (1985).    
    
Messmer, C. H. & Kagi, J. H. Tryptophan residues of creatine kinase: a fluorescence study. Biochemistry 24, 7172–8 (1985).    
    
Munger, K., Germann, U. A., Beltramini, M., Niedermann, D., Baitella-Eberle, G., Kagi, J. H. & Lerch, K. (Cu,Zn)-metallothioneins from fetal bovine liver. Chemical and spectroscopic properties. J. Biol. Chem260, 10032–8 (1985).    
    
Neuhaus, D., Wagner, G., Vasak, M., Kagi, J. H. & Wuthrich, K. Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2. Eur. J. Biochem. 151, 257–73 (1985).    
    
Pande, J., Vasak, M. & Kagi, J. H. Interaction of lysine residues with the metal thiolate clusters in metallothionein. Biochemistry 24, 6717–22 (1985).    
   
Kagi, J. H., Vasak, M., Lerch, K., Gilg, D. E., Hunziker, P., Bernhard, W. R. & Good, M. Structure of mammalian metallothionein. Environ. Health Perspect54, 93–103 (1984).    
    
Neuhaus, D., Wagner, G., Vasak, M., Kagi, J. H. & Wuthrich, K. 113CD-1H spin-spin couplings in homonuclear 1H correlated spectroscopy of metallothionein. Identification of the cysteine 1H spin systems. Eur. J. Biochem. 143, 659–67 (1984).    
    
Vasak, M., Berger, C. & Kagi, J. H. Dynamic structure of metallothionein. FEBS Lett. 168, 174–8 (1984).    
    
Klauser, S., Kagi, J. H. & Wilson, K. J. Characterization of isoprotein patterns in tissue extracts and isolated samples of metallothioneins by reverse-phase high-pressure liquid chromatography. Biochem. J. 209, 71–80 (1983).    
    
Kagi, J., Coombs, T. L., Overnell, J. & Webb, M. Synthesis and function of metallothioneins. Nature 292, 495–6 (1981).    
    
Vasak, M. & Kagi, J. H. Metal thiolate clusters in cobalt(II)-metallothionein. Proc. Natl. Acad. Sci. U. S. A78, 6709–13 (1981).    
    
Vasak, M., Kagi, J. H. & Hill, H. A. Zinc(II), cadmium(II), and mercury(II) thiolate transitions in metallothionein. Biochemistry 20, 2852–6 (1981).    
    
Vasak, M., Kagi, J. H., Holmquist, B. & Vallee, B. L. Spectral studies of cobalt (II)- and Nickel (II)-metallothionein. Biochemistry 20, 6659–64 (1981).    
    
Vasak, M., Galdes, A., Hill, H. A., Kagi, J. H., Bremner, I. & Young, B. W. Investigation of the structure of metallothioneins by proton nuclear magnetic resonance spectroscopy. Biochemistry 19, 416–25 (1980).

Bethune, J. L., Budreau, A. J., Kagi, J. H. & Vallee, B. L. Determination of the charge of horse kidney metallothionein by free boundary electrophoresis. Experientia Suppl. 34, 207–10 (1979).    
    
Buhler, R. H. & Kagi, J. H. Spectroscopic properties of zinc-metallothionein. Experientia Suppl. 34, 211–20 (1979).    
    
Galdes, A., Hill, H. A., Kagi, J. H., Vasak, M., Bremner, I. & Young, B. W. The 1H N.M.R. spectra of metallothioneins. Experientia Suppl. 34, 241–8 (1979).    
    
Kagi, J. H. & Kojima, Y. Nomenclature of metallothionein: a proposal. Experientia Suppl. 34, 141–2 (1979).    
    
Kagi, J. H., Kojima, Y., Kissling, M. M. & Lerch, K. Metallothionein: an exceptional metal thiolate protein. Ciba Found. Symp., 223–37 (1979).    
    
Kissling, M. M., Berger, C., Kagi, J. H., Andersen, R. D. & Weser, U. The amino-terminal sequence of a rat liver metallothionein (MT-2). Experientia Suppl. 34, 181–5 (1979).    
    
Kissling, M. M. & Kagi, J. H. Amino acid sequence of human hepatic metallothioneins. Experientia Suppl. 34, 145–51 (1979).    
    
Kojima, Y., Berger, C. & Kagi, J. H. The amino acid sequence of equine metallothioneins. Experientia Suppl. 34, 153–61 (1979).    
    
Mertens, M. L. & Kagi, J. H. A graphical correction procedure for inner filter effect in fluorescence quenching titrations. Anal. Biochem. 96, 448–55 (1979).    
    
Vasak, M., Nagayama, K., Wuthrich, K., Mertens, M. L. & Kagi, J. H. Creatine kinase. Nuclear magnetic resonance and fluorescence evidence for interaction of adenosine 5'-diphosphate with aromatic residue(s). Biochemistry 18, 5050–5 (1979).

Pfister, K., Kagi, J. H. & Christen, P. Syncatalytic conformational changes in aspartate aminotransferase determined by hydrogen-deuterium exchange. Proc. Natl. Acad. Sci. U. S. A. 75, 145–8 (1978).        

Temler, R. S. & Kagi, J. H. Isolation of human trypsin by affinity chromatography. Enzyme 22, 249–55 (1977).        

Kojima, Y., Berger, C., Vallee, B. L. & Kagi, J. H. Amino-acid sequence of equine renal metallothionein-1B. Proc. Natl. Acad. Sci. U. S. A. 73, 3413–7 (1976).  

     Buhler, R. H. & Kagi, J. H. Human hepatic metallothioneins. FEBS Lett. 39, 229–34 (1974).      

 Kagi, J. H., Himmelhoch, S. R., Whanger, P. D., Bethune, J. L. & Vallee, B. L. Equine hepatic and renal metallothioneins. Purification, molecular weight, amino acid composition, and metal content. J. Biol. Chem. 249, 3537–42 (1974).  

     Kagi, J. H., Li, T. K. & Vallee, B. L. Extrinsic Cotton effects in complexes of creatine phospholinase with adenine coenzymes. Biochemistry 10, 1007–15 (1971).      

 Kagi, J. H. & Ulmer, D. D. Hydrogen-deuterium exchange of cytochrome c. II. Effect of pH. Biochemistry 7, 2718–23 (1968).        

Ulmer, D. D. & Kagi, J. H. Hydrogen-deuterium exchange of cytochrome c. I. Effect of oxidation state. Biochemistry 7, 2710–7 (1968).        

Pulido, P., Kagi, J. H. & Vallee, B. L. Isolation and some properties of human metallothionein. Biochemistry 5, 1768–77 (1966).        

Ulmer, D. D., Kagi, J. H. & Vallee, B. L. Optically active metalloprotein chromophores. I. Metallothionein. Biochem. Biophys. Res. Commun. 8, 327–30 (1962).        

Kagi, J. H. & Vallee, B. L. Metallothionein: a cadmium and zinc-containign protein from equine renal cortex. II. Physico-chemical properties. J. Biol. Chem. 236, 2435–42 (1961).        

Kagi, J. H. & Valee, B. L. Metallothionein: a cadmium- and zinc-containing protein from equine renal cortex. J. Biol. Chem. 235, 3460–5 (1960).        

Kagi, J. H. & Vallee, B. L. The role of zinc in alcohol dehydrogenase. V. The effect of metal-binding agents on thestructure of the yeast alcohol dehydrogenase molecule. J. Biol. Chem. 235, 3188–92 (1960).        

Vallee, B. L. & Kagi, J. H. Zur Bedeutung des Zinks im Stoffwechsel. Schweiz. Med. Wochenschr. 88, 132–5 (1958).        

Burger, M., Giger, K., Kagi, J. & Langemann, H. Änderung der Adrenalin- und Noradrenalinkonzentration im menschlichen Blutplasma unter Phentolamin (Regitin). Helv. Physiol. Pharmacol. Acta 15, 8–13 (1957).        

Kagi, J. Veränderungen der Adrenalin- und Noradrenalinkonzentrationen im menschlichen Blutplasma wahrend der Aethernarkose. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol 230, 479–88 (1957).        

Kagi, J., Burger, M. & Giger, K. Modifikationen zur Fluorescenzmethode von Weil-Ma1Herbe und Bone zur Bestimmung von Adrenalin und Noradrenalin im menschlichen Blutplasma. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol 230, 470–8 (1957).        

Langemann, H. & Kagi, J. Oxytryptamin- und Oxyindolessigsaurebestimmungen bei einem Fall von Carcinoidsyndrom, nebst einigen anderen Untersuchungen über Oxytryptamin. Klin. Wochenschr. 34, 237–41 (1956).        

Kagi, J. & Langemann, H. Zur Phaochromocytomdiagnostik. Schweiz. Med. Wochenschr. 85, 402–4 (1955).