Initial Xtal Screens

When screening for crystallization conditions, it is important to minimize the chemical redundancy between the screens you choose. It is even more critical when working with membrane proteins where it is desirable to screen the most crystallization space with the limited sample you have.

Janet Newman has created a very useful tool to compare the similarity between commercially available crystallization screens. Access this tool by using the guest account from the C6 server: Comparison of Crystallization Conditions @ C3

The C6 Web Tool: A Resource for the Rational Selection of Crystallization Conditions
Janet Newman, Vincent J. Fazio, Brian Lawson and Thomas S.Peat
Cryst. Growth Des., 2010, 10 (6), pp 2785–2792

Eddie Pryor from Anatrace analyzed commercially available membrane protein crystallization screens using the C6 server and made the raw data available through the CCP4 bulletin board: Protein_Screen_Comparison (XLSX, 14 KB)

screen # screen description
GS001 (XLSX, 20 KB) PEG4000, 0.2M Li2SO4, PEG8000_5-30%, pH-gradient:3~10
GS003 (XLSX, 20 KB) PEG4000, 0.2M Li2SO4, PEG6000_5-30%, pH-gradient:3~10
GS005 (XLSX, 19 KB) PEG4000/PEG8000 5-30%, pH-gradient:3~10
GS006 (XLSX, 19 KB) PEG4000/PEG6000 5-30%, pH-gradient:3~10
GS007 (XLSX, 22 KB) Clear Strategy screen I &II, pH 5.5 & 6.5; (MDL:MD1-14 & MD1-15)
GS008 (XLSX, 22 KB) Clear Strategy screen I ⅈ pH 7.5 & 8.5; (MDL:MD1-14 & MD1-15)
GS020 (XLSX, 20 KB) PEG400 15-45%//PEG4000 5-30%, pH gradient 4.5 ~ 9.4,NaCl 1.0M + 150mM (Membrane Protein - MacKinnon Screen)
GS021 (XLSX, 20 KB) PEG400 15-45%//PEG4000 5-30%, pH gradient 4.5 ~ 9.4, (NH4)2SO4 200 mM; Mg-Acetate 50 mM, (Membrane Protein - MacKinnon Screen)
GS022 (XLSX, 21 KB) PEG400 15-45%//PEG4000 5-30%, pH gradient 4.5 ~ 9.4,K-phosphate // Li-Na-sulfate (Membrane Protein - MacKinnon Screen)
GS023 (XLSX, 20 KB) PEG400 15-45%//PEG4000 5-30%, pH gradient 4.5 ~ 9.4,KCl 500mM // CaCl2 200mM (Membrane Protein - MacKinnon Screen)
GS024 (XLSX, 20 KB) PEG400 15-45%//PEG4000 5-30%, pH gradient 4.5 ~ 9.4, Ammonium-Formate; Zinc Acetate, Potassium Iodide (Membrane Protein - MacKinnon Screen)
GS025 (XLSX, 20 KB) PEG400 15-45%//PEG4000 5-30%, pH gradient 4.5 ~ 9.4,No Addition; Potassium-Sodium-Tartrate (Membrane Protein - MacKinnon Screen)
GS027 (XLSX, 19 KB) JCSG-plus™ MD1-37
GS028 (XLSX, 17 KB) PACT premier™ MD1-29, PACT Buffer Protocols (PDF, 594 KB)
GS031 (XLSX, 19 KB) Structure Screen I & II, (MDL: MD1-01 & MD1-02)
GS032 (XLSX, 21 KB) MemStart & MemSys, (MDL: MD1-21 & MD1-25)
GS036 (XLSX, 23 KB) The Anions Suite (Qiagen:Art#130707)
GS037 (XLSX, 22 KB) The Cation Suite (Qiagen:Art#130708)
GS044 (XLSX, 19 KB) Additive screen: JBScreen Plus (Jenabioscience:CS-507L) customer provide buffer solution~10% more concentrated
GS045 (XLSX, 23 KB) Mem Gold (MDL: MD1-41)
GS046 (XLSX, 21 KB) Peg/Ion screen (Hampton Research: HR2-126 & HR2-098)
GS048 (XLSX, 22 KB) Crystal Screen & Crystal Screen 2: (Hampton research:HR2-110 & HR2-112)
GS055 (XLSX, 763 KB) MIDAS, (MDL:MD1-106), Crystallization conditions with alternative polymeric precipitant, MDL Datasheet (PDF, 244 KB)
GS056 (XLSX, 24 KB) HELIX, (MDL:MD1-68), Crystallization conditions for nucleic acid fragments, double stranded DNA, RNA and ribozymes, MDL Datasheet (PDF, 244 KB)
GS066 (XLSX, 20 KB) PEG400 10-45%, pH gradient 4.5 ~ 9.4,NaCl 1.0M + 150mM (Membrane Protein - MacKinnon Screen)
GS067 (XLSX, 26 KB) PEG400 10-45%, pH gradient 4.5 ~ 9.4, (NH4)2SO4 200 mM; Mg-Acetate 50 mM, (Membrane Protein - MacKinnon Screen)
GS068 (XLSX, 28 KB) PEG400 10-45%, pH gradient 4.5 ~ 9.4,K-phosphate // Li-Na-sulfate (Membrane Protein - MacKinnon Screen)
GS069 (XLSX, 28 KB) PEG400 10-45%, pH gradient 4.5 ~ 9.4,KCl 500mM // CaCl2 200mM (Membrane Protein - MacKinnon Screen)
GS070 (XLSX, 27 KB) PEG400 10-45%, pH gradient 4.5 ~ 9.4, Ammonium-Formate; Zinc Acetate, Potassium Iodide (Membrane Protein - MacKinnon Screen)
GS071 (XLSX, 27 KB) PEG400 10-45%, pH gradient 4.5 ~ 9.4,No Addition; Potassium-Sodium-Tartrate (Membrane Protein - MacKinnon Screen)
GS072 (XLSX, 21 KB) MemMeso™- optimized screen for LCP crystallization, Molecular Dimensions MD1-85
GS073 (XLSX, 25 KB) Index HT; Hampton Research HR2-144
GS074 (XLSX, 21 KB) SG1 (ShotGun Screen), Molecular Dimensions MD1-88 (PDF, 176 KB), set of conditions that occur with highest, non redundant frequency amongst all PDB deposit. A drunken search in crystallization space, Vincent J. Fazio,a Thomas S. Peata and Janet Newman
GS075 (XLSX, 19 KB) Morpheus, Molecular Dimensions, MD1-46; Datasheet-GS075 (PDF, 676 KB)
GS076 (XLSX, 19 KB) Pi-PEG, Jena Bioscience, CS-128
GS078 (XLSX, 20 KB) Additiv Screen; Hampton Research, HR2-138
GS079 (XLSX, 19 KB) Gras 1;Hampton Research; HR2-451; different short PEG in low concentration with different salt, pH: 4-9
GS080 (XLSX, 19 KB) Gras 3; Hampton Research, HR2-453; different short PEG in low concentration with different salt, pH: 4-9
GS081 (XLSX, 20 KB) Gras 5; Hampton Research, HR2-455; different short PEG in low concentration with different salt, pH: 4-9
GS083 (XLSX, 24 KB) BCS Screen,
Molecular Dimensions, MD1-104
GS084 (XLSX, 24 KB) Crystal Screen 1+2 Cryo,
Hampton Research; HR2-122 + HR2-121
GS085 (XLSX, 24 KB) (NH4)2SO4 0.5-3.0M, pH-gradient:3~10
GS086 (XLSX, 18 KB) Small Molecule Anion screen (PDF, 665 KB) is a crystallisation screen for organic salts, active pharmaceutical ingredients, coordination complexes and peptides.
Molecular Dimensions MD1-125
GS087 (XLSX, 26 KB) Morpheus Fusion (PDF, 3 MB) is a incomplete factorial protein crystallization screen for membrane and soluble proteins.
Molecular Dimensions MD1-129