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Department of Biochemistry

Publications of the Seeger Group

Marino, J., Hohl, M., Seeger, MA., Zerbe, O. & Geertsma, ER. Bicistronic mRNAs to enhance membrane protein overexpression. J. Mol. Biol. 427, 943-54 (2015). PMID: 25451035.

Hohl, M., Hürlimann, LM., Böhm, S., Schöppe, J., Grütter, MG., Bordignon, E. & Seeger, MA. Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter. Proc. Natl. Acad. Sci. U.S.A. 111, 11025-11030 (2014). PMID: 25030449.

Seeger, MA., Zbinden, R., Flütsch, A., Gutte, PG., Engeler, S., Roschitzki-Voser, H. & Grütter, MG. Design, construction and characterization of a second-generation DARPin library with reduced hydrophobicity. Protein Sci. 22, 1239-1257 (2013).

Eicher, T., Cha, HJ., Seeger, MA., Brandstätter, L., El-Delik, J., Bohnert, J., Kern, WV., Verrey, F., Grütter, MG., Diederichs, K. & Pos, KM. Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop. PNAS  109, 5687-5692 (2012).

Hohl, M., Briand, C., Grütter, MG. & Seeger, MA. Crystal structure of a herterodimeric ABC transporter in its inward-facing conformation. Nat. Struct. Mol. Biol  19, 395-402 (2012).

Mittal, A., Boehm, S., Grütter, MG., Bordignon, E. & Seeger, MA. Asymmetry in the homodimeric ABC transporter MsbA recognized by a DARPin. J Biol Chem 287, 20395-20406 (2012).

Seeger, MA., Mittal, A., Velamakanni, S., Hohl, M., Schauer, S., Salaa, I., Grütter, MG. & van Veen, HW. Tuning the drug efflux activity of an ABC transporter in vivo by in vitro selected DARPin binders. PLoS One 7(6):e37845 (2012).

Brandstätter, L., Sokolova, L., Eicher, T., Seeger, MA., Briand, C., Cha, HJ., Cernescu, M., Bohnert, J., Kern WV., Brutschy, B. & Pos, KM. Analysis of AcrB and AcrB/DARPin ligand complexes by LILBID MS. Biochim Biophys Acta 1808, 2189-2196 (2011).

Monroe, N., Sennhauser, G., Seeger, M., Briand, C. & Grütter, MG. Designed ankyrin repeat protein binders for the crystallization of AcrB: Plasticity of the dominant interface. J. Struct. Biol. 174, 269-281 (2011).

Barrera, NP., Isaacson, SC., Zhou, M., Bavro, VN., Welch, A., Schaedler, TA., Seeger, MA., Miguel, RN., Korkhov, VM., van Venn, HW., Venter, H., Walmsley, AR., Tate, CG. & Robinson, CV. Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions. Nat Methods 6, 585-587 (2009).

Seeger, MA., von Ballmoos, C., Verrey, F. & Pos, KM. Crucial role of Asp408 in the proton translocation pathway of multidrug transporter AcrB: evidence from site-directed mutagenesis and carbodiimide labeling. Biochemistry 48, 5801-5812 (2009).

Seeger, MA. & van Veen, HW. Molecular basis of multidrug transport by ABC transporters. Biochim Biophys Acta 1794, 725-737 (2009).

Velamakanni, S., Lau, CH., Gutmann, DA., Venter, H., Barrera, NP., Seeger, MA., Woebking, B., Matak-Vinkovic, D., Balakrishnan, L., Yao, Y.,U, EC., Shilling, RA., Robinson, CV., Thorn, P. & van Veen, HW. A multidrug ABC transporter with a taste for salt. PLoS One 4, e6137 (2009).

Bohnert, JA., Schuster, S., Seeger, MA., Fahnrich, E., Pos, KM. & Kern, WV. Site-directed mutagenesis reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB. J Bacteriol. 190, 8225-8229 (2008).

Seeger, MA., von Ballmoos, C., Eicher, T., Brandstätter, L., Verrey, F., Diederichs, K. & Pos, KM. Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB. Nat  Struct Mol Biol. 15, 199-205 (2008).

Seeger, MA., Diederichs, K.,  Eicher, T., Brandstätter, L., Schiefner, A., Verrey, F. & Pos, KM. The AcrB efflux pump: conformational cycling and peristalsis lead to multidrug resistance. Curr Drug Targets 9, 729-749 (2008).

Woebking, B., Velamakanni, S., Federici, L., Seeger, MA., Murakami, S. & van Veen, HW. Functional role of transmembrane helix 6 in drug binding and transport by the ABC transporter MsbA. Biochemistry 47, 10904-10914 (2008).

Seeger, MA., Schiefner, A., Eicher, T., Verrey, F., Diederichs, K. & Pos, KM. Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism. Science 313, 1295-1298 (2006). 

Pos, KM., Schiefner, A., Seeger, MA. & Diederichs, K. Crystallographic analysis of AcrB. FEBS Lett. 564, 333-339 (2004).

Van Beilen, JB., Mourlane, F., Seeger, MA., Kovac, J., Li, Z., Smits, TH., Fritsche, U. & Witholt, B. Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and Rhodococcus using polymerase chain reaction with highly degenerative primers. Environ Microbiol. 5, 174-182 (2003).

Smits, TH., Seeger, MA., Witholt, B. & van Beilen, JB. New alkane-responsive expression vectors for Escherichia coli and Pseudomonas. Plasmid 46, 16-24 (2001).