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Department of Biochemistry

Publications of the Plückthun Group 1981-1994 (in alphabetical order)

Freund, C., Ross, A., Plückthun, A. & Holak, T. A. Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy. Biochemistry 33, 3296-3303 (1994).    
Harris, J. R., Plückthun, A. & Zahn, R. Transmission electron microscopy of GroEL, GroES, and the symmetrical GroEL/ES complex. J. Struct. Biol. 112, 216-230 (1994).    
Knappik, A. & Plückthun, A. An improved affinity tag based on the FLAG® peptide for the detection and purification of recombinant antibody fragments. Biotechniques 17, 754-761 (1994).    
Plückthun, A. in Recombinant microbes for industrial and agricultural applications (eds. Murooka, Y. & Imanaka, T.) 233-252 (Marcel Dekker Inc., New York, 1994).    
Plückthun, A. in The pharmacology of monoclonal antibodies (eds. Rosenberg, M. & Moore, G. P.) 269-315 (Springer Verlag, Berlin, 1994).    
Plückthun, A. in Immunochemistry (eds. van Oss, C. J. & van Regenmortel, M. H. V.) 201-236 (Marcel Dekker, Inc., New York, 1994).    
Steipe, B., Schiller, B., Plückthun, A. & Steinbacher, S. Sequence statistics reliably predict stabilizing mutations in a protein domain. J. Mol. Biol. 240, 188-192 (1994).

Virnekäs, B., Ge, L., Plückthun, A., Schneider, K. C., Wellnhofer, G. & Moroney, S. E. Trinucleotide phosphoramidites: Ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. Nucleic Acids Res. 22, 5600-5607 (1994).    
Wülfing, C., Lombardero, J. & Plückthun, A. An Escherichia coli protein consisting of a domain homologous to FK506-binding proteins (FKBP) and a new metal binding motif. J. Biol. Chem. 269, 2895-2901 (1994).    
Wülfing, C. & Plückthun, A. Correctly folded T-cell receptor fragments in the periplasm of Escherichia coli. Influence of folding catalysts. J. Mol. Biol. 242, 655-669 (1994).    
Wülfing, C. & Plückthun, A. Protein folding in the periplasm of Escherichia coli. Mol. Microbiol. 12, 685-692 (1994).    
Zahn, R., Axmann, S. E., Rücknagel, K. P., Jaeger, E., Laminet, A. A. & Plückthun, A. Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. I. GroEL recognizes the signal sequences of ?-lactamase precursor. J. Mol. Biol. 242, 150-164 (1994).    
Zahn, R. & Plückthun, A. Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. II. GroEL recognizes thermally unfolded mature ?-lactamase. J. Mol. Biol. 242, 165-174 (1994).    
Zahn, R., Spitzfaden, C., Ottiger, M., Wüthrich, K. & Plückthun, A. Destabilization of the complete protein secondary structure on binding to the chaperone GroEL. Nature 368, 261-265 (1994).    

Brecht, M., Kellermann, J. & Plückthun, A. Cloning and sequencing of glutamate mutase component E from Clostridium tetanomorphum. FEBS Lett. 319, 84-89 (1993).    
Freund, C., Ross, A., Guth, B., Plückthun, A. & Holak, T. A. Characterization of the linker peptide of the single-chain Fv fragment of an antibody by NMR spectroscopy. FEBS Lett. 320, 97-100 (1993).    
Knappik, A., Krebber, C. & Plückthun, A. The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in Escherichia coli. Bio/Technology 11, 77-83 (1993).    
Pack, P., Kujau, M., Schroeckh, V., Knüpfer, U., Wenderoth, R., Riesenberg, D. & Plückthun, A. Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli. Bio/Technology 11, 1271-1277 (1993).    
Plückthun, A. in Methods of Immunological Analysis (eds. Masseyeff, R. F., Albert, W. H. & Staines, N. A.) 413-430 (VCH Verlagsgesellschaft mbH, Weinheim, 1993).    
Plückthun, A. in Bioorganic Chemistry Frontiers (eds. Dugas, H. & Schmidtchen, F. P.) 25-66 (Springer Verlag, Berlin, 1993).    
Plückthun, A. in Stability and Stabilization of Enzymes (eds. van den Tweel, W. J. J., Harder, A. & Buitelaar, R. M.) 81-90 (Elsevier Science Publishers B.V., 1993).    
Plückthun, A., Pack, P., Knappik, A., Freund, C. & Holak, T. Monovalent single-chain antibodies and bivalent miniantibodies from E. coli: Structure and properties. Ann. Biol. Clin51, 553 (1993).    
Wülfing, C. & Plückthun, A. A versatile and highly repressible Escherichia coli expression system based on invertible promoters: Expression of a gene encoding a toxic product. Gene 136, 199-203 (1993).    
Zahn, R., Harris, J. R., Pfeifer, G., Plückthun, A. & Baumeister, W. Two-dimensional crystals of the molecular chaperone GroEL reveal structural plasticity. J. Mol. Biol. 229, 579-584 (1993).    
Glockshuber, R., Schmidt, T. & Plückthun, A. The disulfide bonds in antibody variable domains: Effects on stability, folding in vitro, and functional expression in Escherichia coli. Biochemistry 31, 1270-1279 (1992).    
Lindner, P., Guth, B., Wülfing, C., Krebber, C., Steipe, B., Müller, F. & Plückthun, A. Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: A Comparison of proteins and protocols. Methods: A Companion to Methods in Enzymology 4, 41-56 (1992).    
Pack, P., Knappik, A., Krebber, C. & Plückthun, A. in Harnessing Biotechnology for the 21st Century (eds. Ladisch, M. R. & Bose, A.) 10-13 (American Chemical Society, Washington, DC, 1992).    
Pack, P. & Plückthun, A. Miniantibodies: Use of amphipathic helices to produce functional, flexibly linked dimeric Fv fragments with high avidity in Escherichia coli. Biochemistry 31, 1579-1584 (1992).    
Plückthun, A. Mono- and bivalent antibody fragments produced in Escherichia coli: Engineering, folding and antigen binding. Immunol. Rev130, 151-188 (1992).    
Steipe, B., Plückthun, A. & Huber, R. Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant. J. Mol. Biol. 225, 739-753 (1992).    
Zahn, R. & Plückthun, A. GroE prevents the accumulation of early folding intermediates of pre-?-lactamase without changing the folding pathway. Biochemistry 31, 3249-3255 (1992).    

2013998Laminet, A. A., Kumamoto, C. A. & Plückthun, A. Folding in vitro and transport in vivo of pre-?-lactamase are SecB independent. Mol. Microbiol. 5, 117-122 (1991).    
Glockshuber, R., Stadlmüller, J. & Plückthun, A. Mapping and modification of an antibody hapten binding site: A site-directed mutagenesis study of McPC603. Biochemistry 30, 3049-3054 (1991).    
Köhler, J., Kikuno, R. & Plückthun, A. in Molecules in natural science and medicine. An Encomium for Linus Pauling (eds. Maksic, Z. B. & Eckert-Maksic, M.) 441-465 (Ellis Horwood, New York, 1991).    
Plückthun, A. Antibody engineering: Advances from the use of Escherichia coli expression systems. Bio/Technology 9, 545-551 (1991).    
Plückthun, A. Strategies for the expression of antibody fragments in Escherichia coli. Methods 2, 88-96 (1991).    
Plückthun, A. Biotechnological aspects of antibody production in E. coli. Acta Biotechnologica 11, 449-456 (1991).    
Plückthun, A. Jahresrückblick Biochemie und Molekularbiologie; Proteinforschung. Nachr. Chem. Tech. Lab39, 178-182 (1991).    
Plückthun, A. Antibody Engineering. Curr. Opin. Biotechnol2, 238-246 (1991).    
Plückthun, A. Chemie mit Genen und Computern? "Chemie heute", Fonds der chemischen Industrie Frankfurt, 60-64 (1991).    
Plückthun, A. & Ge, L. Wie rational ist Zufalls-Screening? - Effiziente Methoden der Selektion von Peptid- und Oligonucleotid-Liganden. Angew. Chem103, 301-303 (1991).    
Plückthun, A. & Pfitzinger, I. Comparison of the Fv fragments of different phosphorylcholine binding antibodies expressed in Escherichia coli. Ann. N. Y. Acad. Sci. 646, 115-124 (1991).

Plückthun, A. & Stadlmüller, J. Catalytic antibodies: Contributions from engineering and expression in Escherichia coli. Ciba Foundation Symposium 159, 103-112; discussion 112-117 (1991).    
Skerra, A., Pfitzinger, I. & Plückthun, A. The functional expression of antibody Fv fragments in Escherichia coli: Improved vectors and a generally applicable purification technique. Bio/Technology 9, 273-278 (1991).    
Skerra, A. & Plückthun, A. Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: Influence of disulphides and cis-prolines. Protein Eng. 4, 971-979 (1991).    
Ganter, C. & Plückthun, A. Glycine to alanine substitutions in helices of glyceraldehyde-3-phosphate dehydrogenase: Effects on stability. Biochemistry 29, 9395-9402 (1990).    
Glockshuber, R., Malia, M., Pfitzinger, I. & Plückthun, A. A comparison of strategies to stabilize immunoglobulin Fv-fragments. Biochemistry 29, 1362-1367 (1990).    
Glockshuber, R., Steipe, B., Huber, R. & Plückthun, A. Crystallization and preliminary X-ray studies of the VL domain of the antibody McPC603 produced in Escherichia coli. J. Mol. Biol. 213, 613-615 (1990).    
Holland, I. B., Kenny, B., Steipe, B. & Plückthun, A. Secretion of heterologous proteins in Escherichia coli. Methods Enzymol182, 132-143 (1990).    
Laminet, A. A., Ziegelhoffer, T., Georgopoulos, C. & Plückthun, A. The Escherichia coli heat shock proteins GroEL and GroES modulate the folding of the ?-lactamase precursor. EMBO J. 9, 2315-2319 (1990).    
Plückthun, A. Antibodies from Escherichia coli. Nature 347, 497-498 (1990).    
Plückthun, A. Wege zu neuen Enzymen: Protein Engineering und Katalytische Antikörper. Chemie in unserer Zeit 24, 182-198 (1990).    
Plückthun, A. Jahresrückblick Physikal.Chemie/Biochemie und Molekularbiologie. Nachr. Chem. Tech. Lab. 38, 216-222 (1990).    
Plückthun, A. Recombinant antibodies produced in E.coli: Prospects for catalysis. Fresenius J. Anal. Chem337, 13 (1990).    
Plückthun, A. Towards new enzymes: Protein engineering and catalytic antibodies. Kontakte (Darmstadt), 40-59 (1990).    
Plückthun, A. in 40 Jahre Fonds der Chemischen Industrie. Wissenschaftliche Beiträge von Dozenten-Stipendiaten 187-196 (1990).

Plückthun, A. in 6th International Symposium on genetics of industrial microorganisms (GIM 90) (eds. Heslot, H. et al.) 17-23 (Strasbourg, 1990).    
Plückthun, A., Glockshuber, R., Skerra, A. & Stadlmüller, J. Properties of Fv and Fab fragments of the antibody McPC603 expressed in E. coli. Behring Institute Mitt., 48-55 (1990).    
Plückthun, A., Glockshuber, R., Skerra, A. & Stadlmüller, J. Engineering of Fv and Fab fragments of the antibody McPC603 expressed in E. coli. ICSU Short Rep. 10, 94-95 (1990).    
Plückthun, A. & Köhler, J. in Forum '90 Wissenschaft und Technik. Informatik Fachberichte (eds. Friemel, H.-J., Müller-Schönberger, G. & Schütt, A.) 258-274 (Springer-Verlag, Trier, 1990, Proceedings, 1990).    
Skerra, A., Glockshuber, R. & Plückthun, A. Structural features of the McPC603 Fab fragment not defined in the X-ray structure. FEBS Lett. 271, 203-206 (1990).    

Ellinger, S., Glockshuber, R., Jahn, G. & Plückthun, A. Cleavage of procaryotically expressed human immunodeficiency virus fusion proteins by factor Xa and application in western blot (immunoblot) assays. J. Clin. Microbiol. 27, 971-976 (1989).    
Hörz, W., Kröger, M., Kuschmitz, D. & Plückthun, A. in Biotechnology: A comprehensive treatise (eds. Rehm, H.-J. & Reed, G.) 530-552 (VCH Verlagsgesellschaft, Weinheim, 1989).    
Laminet, A. A. & Plückthun, A. The precursor of ?-lactamase: Purification, properties and folding kinetics. EMBO J. 8, 1469-1477 (1989).    
Plückthun, A. & Skerra, A. Expression of functional antibody Fv and Fab fragments in Escherichia coli. Methods Enzymol. 178, 497-515 (1989).    
Plückthun, A., Stadlmüller, J., Skerra, A., Glockshuber, R. & Steipe, B. Bacterial expression and characterization of antigen binding fragments of the antibody McPC603. J. Cell. Biochem13A, 92 (1989).    
Plückthun, A. & Pfitzinger, I. Membrane-bound ?-lactamase forms in Escherichia coli. J. Biol. Chem263, 14315-14322 (1988).    
Plückthun, A., Skerra, A., Glockshuber, R. & Stadlmüller, J. in Protein structure and protein engineering (eds. Winnacker, E.-L. & Huber, R.) 123-131 (Springer Verlag, Berlin, Heidelberg, 1988).    
Plückthun, A., Skerra, A., Glockshuber, R., Stadlmüller, J. & Pfitzinger, I. in Biological Chemistry Hoppe-Seyler 206-207 (1988).    
Skerra, A. & Plückthun, A. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 240, 1038-1041 (1988).    
Plückthun, A., Glockshuber, R., Pfitzinger, I., Skerra, A. & Stadlmüller, J. Engineering of antibodies with a known three-dimensional structure. Cold Spring Harbor Symp. Quant. Biol. 52, 105-112 (1987).    
Plückthun, A., Glockshuber, R., Stadlmüller, J. & Skerra, A. Synthetic antibodies with known 3-D structure. J. Cell. Biochem. 11C, 236 (1987).    
Plückthun, A. & Knowles, J. R. The consequences of stepwise deletions from the signal-processing site of  ?-lactamase. J. Biol. Chem262, 3951-3957 (1987).    

Dennis, E. A. & Plückthun, A. in Enzymes of lipid metabolism II (eds. Freysz, L., Dreyfus, H., Massarelli, R. & Gatt, S.) 121-132 (Plenum Press, New York, 1986).    
Fanni, T., Lombardo, D., Plückthun, A. & Dennis, E. A. Rate determining step in phospholipase A2 mechanism: 18O Isotope exchange determined by 13C NMR. Fed. Proc45, 1557 (1986).    
Glockshuber, R., Mertz, R., Stadlmüller, J. & Plückthun, A. Engineering of antibody variable domains with known structure. Biological Chemistry Hoppe-Seyler 367 Suppl., 164 (1986).    
Lombardo, D., Fanni, T., Plückthun, A. & Dennis, E. A. Rate-determining step in phospholipase A2 mechanism. 18O isotope exchange determined by 13C NMR. J. Biol. Chem. 261, 11663-11666 (1986).    
Plückthun, A., DeBony, J., Fanni, T. & Dennis, E. A. Conformation of fatty acyl chains in ?- and ?-phosphatidylcholine and phosphatidylethanolamine derivatives in sonicated vesicles. Biochim. Biophys. Acta 856, 144-154 (1986).    
Kadonaga, J. T., Plückthun, A. & Knowles, J. R. Signal sequence mutants of ?-lactamase. J. Biol. Chem260, 16192-16199 (1985).    
Plückthun, A. & Dennis, E. A. Activation, aggregation, and product inhibition of cobra venom phospholipase A2 and comparison with other phospholipases. J. Biol. Chem260, 11099-11106 (1985).    
Plückthun, A., Rohlfs, R., Davidson, F. F. & Dennis, E. A. Short-chain phosphatidylethanolamines: Physical properties and susceptibility of the monomers to phospholipase A2 action. Biochemistry 24, 4201-4208 (1985).    
Dennis, E. A. & Plückthun, A. in Phosphorus-31 NMR, Principles and Applications (ed. Gorenstein, D. G.) 423-447 (Academic Press Inc., 1984).    

Vogel, C. W., Plückthun, A., Podack, E. R., Dennis, E. A. & Müller-Eberhard, H. J. The membrane attack complex of complement and its precursor proteins lack phospholipase activity. Mol. Immunol20, 377-382 (1983).    

Plückthun, A., deBony, J. & Dennis, E. A. 1H-NMR Investigation of the conformation of charged and zwitterionic ? -and ?-phospholipids in sonicated vesicles. Biophys. J37, 13a (1982).    
Plückthun, A. & Dennis, E. A. Role of monomeric activators in cobra venom phospholipase A2 action. Biochemistry 21, 1750-1756 (1982).    
Plückthun, A. & Dennis, E. A. Acyl and phosphoryl migration in lysophospholipids: Importance in phospholipid synthesis and phospholipase specificity. Biochemistry 21, 1743-1750 (1982).    

Dennis, E. A., Darke, P. L., Deems, R. A., Kensil, C. R. & Plückthun, A. Cobra venom phospholipase A2: A review of its action toward lipid/water interfaces. Mol. Cell. Biochem36, 37-45 (1981).    
Plückthun, A. & Dennis, E. A. 31P Nuclear magnetic resonance study on the incorporation of monomeric phospholipids into nonionic detergent micelles. J. Phys. Chem85, 678-683 (1981).    
Plückthun, A. & Dennis, E. A. Phospholipid activation of phospholipase A2 from cobra venom. Fed. Proc40, 1805 (1981).    
Plückthun, A. & Dennis, E. A. Phospholipase A2 hydrolysis of phospholipids: Use of 31P NMR to study the hydrolysis, acyl migration, regiospecific synthesis, and solubilization of phospholipids. Phosphorus Chemistry, ACS Symposium Series 171, 591-594 (1981).    
Vogel, C. W., Plückthun, A., Müller-Eberhard, H. J. & Dennis, E. A. Hemolytic assay for venom phospholipase A2. Anal. Biochem118, 262-268 (1981).